Antibodies, Proteins, Kits and Reagents for Life Science

251 280 ₸ Product size

100 µl 251 280 ₸

Order now and get it on Wednesday March 10, 2021

Anti-PRSS1 antibody [EPR19497] (ab200996)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19497] to PRSS1
Suitable for: IHC-P, WB
Reacts with: Human, Recombinant fragment

Product name

Anti-PRSS1 antibody [EPR19497]
See all PRSS1 primary antibodies
Description

Rabbit monoclonal [EPR19497] to PRSS1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

Recombinant fragment

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human PRSS1 fragment recombinant protein; Human pancreas lysate. IHC-P: Human pancreas tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human Recombinant fragment

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19497
Isotype

IgG
Research areas

Western blot - Anti-PRSS1 antibody [EPR19497] (ab200996)

All lanes : Anti-PRSS1 antibody [EPR19497] (ab200996) at 1/1000 dilution

Lane 1 : Human PRSS1 fragment recombinant protein
Lane 2 : Human TRY3 full length recombinant protein
Lane 3 : Human TRY6 full length recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 27 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 1 second; Lane 2 and 3: 3 minutes.

Human PRSS1 fragment recombinant protein contains aa24-122 with a His-Tag^®. Human TRY3 full length recombinant protein contains aa1-304 with a His-Tag^®. Human TRY6 full length recombinant protein contains aa1-247 with a His-Tag^®. These three recombinant proteins were made in-house.
Western blot - Anti-PRSS1 antibody [EPR19497] (ab200996)

Anti-PRSS1 antibody [EPR19497] (ab200996) at 1/1000 dilution + Human TRY2 full length recombinant protein at 0.01 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 27 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

Human TRY2 full length recombinant protein contains aa1-247 with a GST-Tag. It was made in-house.
Western blot - Anti-PRSS1 antibody [EPR19497] (ab200996)

All lanes : Anti-PRSS1 antibody [EPR19497] (ab200996) at 1/5000 dilution

Lane 1 : Human pancreas lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human fetal heart lysate
Lane 4 : Human fetal kidney lysate
Lane 5 : Human fetal spleen lysate
Lane 6 : Human fetal lung lysate
Lane 7 : BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate
Lane 8 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
Lane 9 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

Trypsin 1, encoded by the PRSS1 gene, is synthesized in the pancreas. The expression profile observed is consistent with what has been described in the literatures (PMID: 3011602, 7945238, 24722290).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS1 antibody [EPR19497] (ab200996)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PRSS1 with ab200996 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on acinar cells Human pancreas is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS1 antibody [EPR19497] (ab200996)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PRSS1 with ab200996 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human kidney. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-PRSS1 antibody [EPR19497] (ab200996)

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