Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PRSS1 antibody [EPR19497] (ab200996)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19497] to PRSS1
  • Suitable for: IHC-P, WB
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-PRSS1 antibody [EPR19497]
    See all PRSS1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19497] to PRSS1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human PRSS1 fragment recombinant protein; Human pancreas lysate. IHC-P: Human pancreas tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19497

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PRSS1 antibody [EPR19497] (ab200996)
    Western blot - Anti-PRSS1 antibody [EPR19497] (ab200996)
    All lanes : Anti-PRSS1 antibody [EPR19497] (ab200996) at 1/1000 dilution

    Lane 1 : Human PRSS1 fragment recombinant protein
    Lane 2 : Human TRY3 full length recombinant protein
    Lane 3 : Human TRY6 full length recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 27 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 second; Lane 2 and 3: 3 minutes.

    Human PRSS1 fragment recombinant protein contains aa24-122 with a His-Tag®. Human TRY3 full length recombinant protein contains aa1-304 with a His-Tag®. Human TRY6 full length recombinant protein contains aa1-247 with a His-Tag®.  These three recombinant proteins were made in-house.

  • Western blot - Anti-PRSS1 antibody [EPR19497] (ab200996)
    Western blot - Anti-PRSS1 antibody [EPR19497] (ab200996)
    Anti-PRSS1 antibody [EPR19497] (ab200996) at 1/1000 dilution + Human TRY2 full length recombinant protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 27 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human TRY2 full length recombinant protein contains aa1-247 with a GST-Tag.  It was made in-house.

  • Western blot - Anti-PRSS1 antibody [EPR19497] (ab200996)
    Western blot - Anti-PRSS1 antibody [EPR19497] (ab200996)
    All lanes : Anti-PRSS1 antibody [EPR19497] (ab200996) at 1/5000 dilution

    Lane 1 : Human pancreas lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : Human fetal heart lysate
    Lane 4 : Human fetal kidney lysate
    Lane 5 : Human fetal spleen lysate
    Lane 6 : Human fetal lung lysate
    Lane 7 : BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate
    Lane 8 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
    Lane 9 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Trypsin 1, encoded by the PRSS1 gene, is synthesized in the pancreas. The expression profile observed is consistent with what has been described in the literatures (PMID: 3011602, 7945238, 24722290).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS1 antibody [EPR19497] (ab200996)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS1 antibody [EPR19497] (ab200996)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PRSS1 with ab200996 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on acinar cells Human pancreas is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS1 antibody [EPR19497] (ab200996)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS1 antibody [EPR19497] (ab200996)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PRSS1 with ab200996 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human kidney. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-PRSS1 antibody [EPR19497] (ab200996)
    Anti-PRSS1 antibody [EPR19497] (ab200996)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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