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PBR overexpression 293T lysate (whole cell) (ab94211)

PBR overexpression 293T lysate (whole cell) (ab94211)
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Overview

  • Product name

    PBR overexpression 293T lysate (whole cell)
    See all PBR lysates
  • General notes

    ab94211 is a 293T cell transfected lysate in which Human PBR has been transiently over-expressed using a pCMV-PBR plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes

  • Tested applications

    Suitable for: WBmore details

Properties

  • Mycoplasma free

    Yes
  • Form

    Liquid
  • Storage instructions

    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer

    Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Channels
    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Metabolism
    • Types of disease
    • Cancer
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism
  • Background

    Function: Responsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells. Tissue specificity: Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids. Similarity: Belongs to the TspO/BZRP family.

Images

  • SDS-PAGE - PBR overexpression 293T lysate (whole cell) (ab94211)
    SDS-PAGE - PBR overexpression 293T lysate (whole cell) (ab94211)
    ab94211 at 15μg/lane on an SDS-PAGE gel
  • Western blot - PBR overexpression 293T lysate (whole cell) (ab94211)
    Western blot - PBR overexpression 293T lysate (whole cell) (ab94211)
    All lanes : Anti-PBR antibody (ab54895) at 1/500 dilution

    Lane 1 : PBR overexpression 293T lysate (whole cell) (ab94211)
    Lane 2 : 293T Non Transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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