PBR overexpression 293T lysate (whole cell) (ab94211)
Overview
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Product name
PBR overexpression 293T lysate (whole cell)
See all PBR lysates -
General notes
ab94211 is a 293T cell transfected lysate in which Human PBR has been transiently over-expressed using a pCMV-PBR plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Research areas
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Background
Function: Responsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells. Tissue specificity: Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids. Similarity: Belongs to the TspO/BZRP family.
Images
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ab94211 at 15μg/lane on an SDS-PAGE gel
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All lanes : Anti-PBR antibody (ab54895) at 1/500 dilution
Lane 1 :PBR overexpression 293T lysate (whole cell) (ab94211)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution