Anti-SSR3 antibody - N-terminal (ab190936)
Key features and details
- Rabbit polyclonal to SSR3 - N-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SSR3 antibody - N-terminal
See all SSR3 primary antibodies -
Description
Rabbit polyclonal to SSR3 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Orangutan
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Immunogen
Synthetic peptide corresponding to Human SSR3 aa 1-100 (N terminal).
Database link: Q9UNL2 -
Positive control
- WB: Rat liver, rat spleen, A431, HeLa, U87 and SMMC lysates; IHC-P: Human prostatic cancer, Rat brain and intestine tissues, and Mouse intestine tissue. ICC: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (ab190936)
SSR3 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit ab190936 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (ab190936)Immunohistochemical analysis of paraffin-embedded Rat brain tissue labeling SSR3 with ab190936 at 1 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (ab190936)SSR3 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190936 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (ab190936)SSR3 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190936 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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Immunocytochemistry/ Immunofluorescence - Anti-SSR3 antibody - N-terminal (ab190936)SSR3 was detected in immunocytochemical section of HeLa cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml ab190936 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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Western blot - Anti-SSR3 antibody - N-terminal (ab190936)All lanes : Anti-SSR3 antibody - N-terminal (ab190936) at 0.5 µg/ml
Lane 1 : Rat liver tissue lysate
Lane 2 : Rat spleen tissue lysate
Lane 3 : A431 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : U87 cell lysate
Lane 6 : SMMC cell lysate
Predicted band size: 21 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (ab190936)Immunohistochemical analysis of paraffin-embedded Human prostatic cancer tissue labeling SSR3 with ab190936 at 1 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSR3 antibody - N-terminal (ab190936)Immunohistochemical analysis of paraffin-embedded Rat intestine tissue labeling SSR3 with ab190936 at 1 µg/ml.
