Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Carbonic Anhydrase 4/CA4 antibody [C1] (ab239505)

Key features and details

  • Mouse monoclonal [C1] to Carbonic Anhydrase 4/CA4
  • Suitable for: IHC-P, WB
  • Reacts with: Rat
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Carbonic Anhydrase 4/CA4 antibody [C1]
    See all Carbonic Anhydrase 4/CA4 primary antibodies

  • Description

    Mouse monoclonal [C1] to Carbonic Anhydrase 4/CA4

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Rat

  • Immunogen

    Recombinant fragment (His-T7-tag) corresponding to Rat Carbonic Anhydrase 4/CA4 aa 1-250. Two N-terminal tags, His-tag and T7-tag (Expressed in E.coli).
    Database link: P48284

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Rat skeletal muscle tissue. WB: Rat lung and brain lysates; Recombinant rat Carbonic Anhydrase IV/CA4 protein.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    Purified from TCS.

  • Clonality

    Monoclonal

  • Clone number

    C1

  • Isotype

    IgG2b

  • Light chain type

    kappa

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 4/CA4 antibody [C1] (ab239505)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 4/CA4 antibody [C1] (ab239505)

    Paraffin-embedded rat skeletal muscle tissue stained for Carbonic Anhydrase 4/CA4 using ab239505 at 30 µg/ml in immunohistochemical analysis. DAB staining.

  • Western blot - Anti-Carbonic Anhydrase 4/CA4 antibody [C1] (ab239505)
    Western blot - Anti-Carbonic Anhydrase 4/CA4 antibody [C1] (ab239505)
    All lanes : Anti-Carbonic Anhydrase 4/CA4 antibody [C1] (ab239505) at 2 µg/ml

    Lane 1 : Rat lung lysate
    Lane 2 : Rat brain lysate

    Secondary
    All lanes : HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution

    Predicted band size: 35 kDa

  • Western blot - Anti-Carbonic Anhydrase 4/CA4 antibody [C1] (ab239505)
    Western blot - Anti-Carbonic Anhydrase 4/CA4 antibody [C1] (ab239505)
    Anti-Carbonic Anhydrase 4/CA4 antibody [C1] (ab239505) at 2 µg/ml + Recombinant rat Carbonic Anhydrase IV/CA4 protein

    Secondary
    HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution

    Predicted band size: 35 kDa

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