Anti-AGO4 with anti-GAPDH internal loading control antibody (ab94772)
Key features and details
- Rabbit polyclonal to AGO4 with anti-GAPDH internal loading control
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AGO4 with anti-GAPDH internal loading control antibody -
Description
Rabbit polyclonal to AGO4 with anti-GAPDH internal loading control -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human AGO4 with anti-GAPDH internal loading control aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab138813) -
Positive control
- This cocktail gave a positive signal in both Human Brain and Testis Tissue lysates as well as the following whole cell lysates: HeLa; A431; Jurkat; HepG2.
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General notes
This product was previously labelled as Argonaute 4, Argonaute 4 with anti-GAPDH internal loading control
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatRabbitChimpanzeeMacaque monkeyGorillaChinese hamsterOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 37, 97 kDa (predicted molecular weight: 37, 97 kDa).ab94772 contains 2 antibodies, generated by raising against an AGO4 immunogen and spiking with anti-GAPDH loading control.
Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 37, 97 kDa (predicted molecular weight: 37, 97 kDa).ab94772 contains 2 antibodies, generated by raising against an AGO4 immunogen and spiking with anti-GAPDH loading control.
Application notesIs unsuitable for ChIP,Flow Cyt,ICC/IF or IHC-P.Target
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Function
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for RNA-directed transcription and replication of the human hapatitis delta virus (HDV). -
Sequence similarities
Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
Cellular localization
Cytoplasm > P-body. - Information by UniProt
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Database links
- Entrez Gene: 192670 Human
- SwissProt: Q9HCK5 Human
- Unigene: 471492 Human
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Alternative names
- AGO4_HUMAN antibody
- Argonaute4 antibody
- eIF-2C 4 antibody
see all
Images
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Western blot - Anti-AGO4 with anti-GAPDH internal loading control antibody (ab94772)All lanes : Anti-AGO4 with anti-GAPDH internal loading control antibody (ab94772) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Human brain tissue lysate - total protein (ab29466)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Human testis tissue lysate - total protein (ab30257)
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37, 97 kDa
Observed band size: 37,97 kDa why is the actual band size different from the predicted?
Additional bands at: 52 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minuteThe 97 and 37 kDa bands correlate to AGO4 and GAPDH, respectively.
Datasheets and documents
References (0)
ab94772 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-AGO4 with anti-GAPDH internal loading control antibody (ab94772)All lanes : Anti-AGO4 with anti-GAPDH internal loading control antibody (ab94772) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Human brain tissue lysate - total protein (ab29466)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Human testis tissue lysate - total protein (ab30257)
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37, 97 kDa
Observed band size: 37,97 kDa why is the actual band size different from the predicted?
Additional bands at: 52 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minuteThe 97 and 37 kDa bands correlate to AGO4 and GAPDH, respectively.
