Anti-GAPDH antibody (ab245357)
Key features and details
- Rabbit polyclonal to GAPDH
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GAPDH antibody
See all GAPDH primary antibodies -
Description
Rabbit polyclonal to GAPDH -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide within Human GAPDH aa 300-335. The exact sequence is proprietary. NP_002037.2
Database link: P04406 -
Positive control
- WB: HeLa, HEK-293T, 4T1, CT26.WT and C6 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245357 was affinity purified using an epitope specific to GAPDH immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GAPDH antibody (ab245357)All lanes : Anti-GAPDH antibody (ab245357) at 0.5 µg/ml
Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 3 : 4T1 (Mouse mammary gland carcinoma cell line) whole cell lysate
Lane 4 : CT26.WT (Mouse cell line) whole cell lysate
Lane 5 : C6 (Rat glial tumor cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 36 kDa
Exposure time: 3 minutesDetection: Chemiluminescence.
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Western blot - Anti-GAPDH antibody (ab245357)All lanes : Anti-GAPDH antibody (ab245357) at 0.04 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 36 kDa
Exposure time: 30 secondsDetection: Chemiluminescence.
