HRP Anti-GAPDH antibody [EPR6256] - Loading Control (ab185059)
![HRP Anti-GAPDH antibody [EPR6256] - Loading Control (ab185059)](https://www.abcam.com/ps/products/185/ab185059/Images/ab185059-218726-anti-gapdh-antibody-epr6256-loading-control-hrp-western-blot.jpg "HRP Anti-GAPDH antibody [EPR6256] - Loading Control (ab185059)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR6256] to GAPDH - Loading Control
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-GAPDH antibody [EPR6256] - Loading Control
See all GAPDH primary antibodies -
Description
HRP Rabbit monoclonal [EPR6256] to GAPDH - Loading Control -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 293T, HeLa, HepG2, HUVEC, MCF7 or SH SY5Y cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6256 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-GAPDH antibody [EPR6256] - Loading Control (ab185059)All lanes : HRP Anti-GAPDH antibody [EPR6256] - Loading Control (ab185059) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 2 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab185059 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406
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![HRP Anti-GAPDH antibody [EPR6256] - Loading Control (ab185059)](https://www.abcam.com/ps/products/185/ab185059/Images/ab185059-2-benefits-of-recombinant-antibodies.png)
HRP Anti-GAPDH antibody [EPR6256] - Loading Control (ab185059)
