FITC Anti-GAPDH antibody [EPR16891] (ab224005)
![FITC Anti-GAPDH antibody [EPR16891] (ab224005)](https://www.abcam.com/ps/products/224/ab224005/Images/ab224005-310867-ICCAP38663721.jpg "FITC Anti-GAPDH antibody [EPR16891] (ab224005)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- FITC Rabbit monoclonal [EPR16891] to GAPDH
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
Overview
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Product name
FITC Anti-GAPDH antibody [EPR16891]
See all GAPDH primary antibodies -
Description
FITC Rabbit monoclonal [EPR16891] to GAPDH -
Host species
Rabbit -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Recombinant fragment within Mouse GAPDH aa 100 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P16858 -
Positive control
- ICC/IF: HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16891 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - FITC Anti-GAPDH antibody [EPR16891] (ab224005)
ab224005 staining GAPDH in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab224005 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![FITC Anti-GAPDH antibody [EPR16891] (ab224005)](https://www.abcam.com/ps/products/224/ab224005/Images/ab224005-2-benefits-of-recombinant-antibodies.png)
FITC Anti-GAPDH antibody [EPR16891] (ab224005)
