Anti-COQ10B antibody (ab41997)
Key features and details
- Rabbit polyclonal to COQ10B
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-COQ10B antibody -
Description
Rabbit polyclonal to COQ10B -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Orangutan
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Immunogen
Synthetic peptide corresponding to Human COQ10B aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab41996) -
Positive control
- This antibody gave a positive signal 1n the following lysates: HepG2 Whole Cell HeLa Whole Cell Rat Liver Tissue Mouse Pancreas Tissue Mouse Liver Tissue (data not shown)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab41997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 5 µg/ml. IHC-P Use a concentration of 5 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa). Target
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Relevance
COQ10 (Coenzyme Q10) is found in a wide variety of foods and is synthesized in all tissues. The de novo biosynthesis of COQ10, from its precursor tyrosine, is a multistage process that requires at least eight vitamins and several trace elements. More complex enzymes have an absolute dependancy on 'cofactors', a large group of molecules to which the coenzymes belong, for their catalyitic function. Coenzyme Q10 is the cofactor for, but not limited to, several mitochondrial enzymes that are central to the supply of adenosine triphosphate (ATP). In addition, COQ10 in its reduced form is a potent antioxidant, and in this capacity, a reduction in COQ10 levels are associtated with a variety of degenerative process including skin aging and neurodegenerative conditions such as Alzhiemer's, Huntingdon's and Parkinson's diseases. -
Cellular localization
Mitochondrial -
Database links
- Entrez Gene: 80219 Human
- Entrez Gene: 67876 Mouse
- SwissProt: Q9H8M1 Human
- SwissProt: Q3THF9 Mouse
- SwissProt: Q5I0I9 Rat
- Unigene: 632547 Human
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Alternative names
- Coenzyme Q10 antibody
- Coenzyme Q10 B antibody
- Coenzyme Q10 homolog B antibody
- Coenzyme Q10B antibody
Images
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Western blot - Anti-COQ10B antibody (ab41997)All lanes : Anti-COQ10B antibody (ab41997) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Liver (Rat) Tissue Lysate
Lane 4 :Mouse pancreas tissue lysate - total protein (ab29363)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
COQ10B has a predicted molecular weight of 27 kDa; however as this is a novel protein, it may contain uncharacterised modification sites that affect the migration of the protein. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COQ10B antibody (ab41997)IHC image of ab41997 staining COQ10B in Human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41997, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunocytochemistry/ Immunofluorescence - Anti-COQ10B antibody (ab41997)ICC/IF image of ab41997 stained HeLa cells. The cells were stained with Mitotracker Orange CMTMRos at a concentration of 500nM in PBS and incubated for 45 minutes at 37°C. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41997, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (3)
ab41997 has been referenced in 3 publications.
- Urbano F et al. Atorvastatin but Not Pravastatin Impairs Mitochondrial Function in Human Pancreatic Islets and Rat ß-Cells. Direct Effect of Oxidative Stress. Sci Rep 7:11863 (2017). WB ; Rat . PubMed: 28928397
- Oezel L et al. Fibromyalgia syndrome: metabolic and autophagic processes in intermittent cold stress mice. Pharmacol Res Perspect 4:e00248 (2016). PubMed: 27713820
- Stride N et al. Impaired mitochondrial function in chronically ischemic human heart. Am J Physiol Heart Circ Physiol 304:H1407-14 (2013). PubMed: 23542918
Images
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Western blot - Anti-COQ10B antibody (ab41997)All lanes : Anti-COQ10B antibody (ab41997) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Liver (Rat) Tissue Lysate
Lane 4 :Mouse pancreas tissue lysate - total protein (ab29363)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
COQ10B has a predicted molecular weight of 27 kDa; however as this is a novel protein, it may contain uncharacterised modification sites that affect the migration of the protein. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COQ10B antibody (ab41997)IHC image of ab41997 staining COQ10B in Human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41997, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunocytochemistry/ Immunofluorescence - Anti-COQ10B antibody (ab41997)ICC/IF image of ab41997 stained HeLa cells. The cells were stained with Mitotracker Orange CMTMRos at a concentration of 500nM in PBS and incubated for 45 minutes at 37°C. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41997, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
