Anti-TUG antibody [EPR8616] (ab131217)
![Anti-TUG antibody [EPR8616] (ab131217)](https://www.abcam.com/ps/products/131/ab131217/Images/ab131217-266963-ab131217KOWB.jpg "Anti-TUG antibody [EPR8616] (ab131217)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8616] to TUG
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Overview
-
Product name
Anti-TUG antibody [EPR8616]
See all TUG primary antibodies -
Description
Rabbit monoclonal [EPR8616] to TUG -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
-
Immunogen
Synthetic peptide corresponding to residues on the C-terminus of the Human TUG protein (Q9BZE9).
-
Positive control
- K562, 293T, and SW480 cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8616 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-TUG antibody [EPR8616] (ab131217)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: TUG knockout HAP1 cell lysate (20 µg)
Lane 3: NIH/3T3 cell lysate (20 µg)
Lane 4: HEK293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab131217 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab131217 was shown to specifically react with TUG when TUG knockout samples were used. Wild-type and TUG knockout samples were subjected to SDS-PAGE. ab131217 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-TUG antibody [EPR8616] (ab131217)](https://www.abcam.com/ps/products/131/ab131217/Images/TUG-Primary-antibodies-ab131217-1.jpg)
Western blot - Anti-TUG antibody [EPR8616] (ab131217)All lanes : Anti-TUG antibody [EPR8616] (ab131217) at 1/10000 dilution
Lane 1 : K562 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : SW480 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 60 kDa
-
![Anti-TUG antibody [EPR8616] (ab131217)](https://www.abcam.com/ps/products/131/ab131217/Images/ab131217-3-benefits-of-recombinant-antibodies.png)
Anti-TUG antibody [EPR8616] (ab131217)
