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Anti-TUG antibody [EPR8615] (ab131237)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8615] to TUG
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-TUG antibody [EPR8615]
    See all TUG primary antibodies

  • Description

    Rabbit monoclonal [EPR8615] to TUG

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues in human TUG (Q9BZE9).

  • Positive control

    • K562, 293T, and SW480 cell lysates for Western Blot and Human colon tissue for IHC-P.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TUG antibody [EPR8615] (ab131237)
    Western blot - Anti-TUG antibody [EPR8615] (ab131237)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: TUG knockout HAP1 cell lysate (20 µg)
    Lane 3: NIH/3T3 cell lysate (20 µg)
    Lane 4: HEK293 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab131237 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab131237 was shown to specifically react with TUG when TUG knockout samples were used. Wild-type and TUG knockout samples were subjected to SDS-PAGE. ab131237 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776  secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUG antibody [EPR8615] (ab131237)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUG antibody [EPR8615] (ab131237)

    ab131237, at 1/100, staining TUG in Formalin-fixed, Paraffin-embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-TUG antibody [EPR8615] (ab131237)
    Western blot - Anti-TUG antibody [EPR8615] (ab131237)
    All lanes : Anti-TUG antibody [EPR8615] (ab131237) at 1/10000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : SW480 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 60 kDa

  • OI-RD Scanning - Anti-TUG antibody [EPR8615] (ab131237)
    OI-RD Scanning - Anti-TUG antibody [EPR8615] (ab131237)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-TUG antibody [EPR8615] (ab131237)
    Anti-TUG antibody [EPR8615] (ab131237)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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