Anti-GAPDH antibody [12G11] - Loading Control (ab226408)
![Anti-GAPDH antibody [12G11] - Loading Control (ab226408)](https://www.abcam.com/ps/products/226/ab226408/Images/ab226408-287714-anti-gapdh-antibody-12g11-loading-control-western-blot.jpg "Anti-GAPDH antibody [12G11] - Loading Control (ab226408)")
Key features and details
- Mouse monoclonal [12G11] to GAPDH - Loading Control
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-GAPDH antibody [12G11] - Loading Control
See all GAPDH primary antibodies -
Description
Mouse monoclonal [12G11] to GAPDH - Loading Control -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human GAPDH aa 150-200. The exact sequence is proprietary.
Database link: P04406 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IHC: Human ovarian carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226408 was affinity purified using an epitope specific to GAPDH immobilized on solid support. Purified from TCS. -
Clonality
Monoclonal -
Clone number
12G11 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-GAPDH antibody [12G11] - Loading Control (ab226408)All lanes : Anti-GAPDH antibody [12G11] - Loading Control (ab226408) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 36 kDa
Exposure time: 3 minutesCell lysates were prepared using NETN lysis buffer.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [12G11] - Loading Control (ab226408)](https://www.abcam.com/ps/products/226/ab226408/Images/ab226408-287715-anti-gapdh-antibody-12g11-loading-control-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [12G11] - Loading Control (ab226408)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for GAPDH using ab226408 at 1/20 dilution in immunohistochemical analysis. Detection: DAB staining.
