Astana Biomed Group, an authorized Abcam distributor in Central Asia

HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR16891] to GAPDH - Loading Control
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-GAPDH antibody [EPR16891] - Loading Control
    See all GAPDH primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR16891] to GAPDH - Loading Control

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Fish, Monkey, Zebrafish, Xenopus tropicalis

  • Immunogen

    Recombinant fragment within Mouse GAPDH aa 100 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P16858

  • Positive control

    • WB: HeLa, NIH3T3, PC-12 whole cell lysates and Human, Mouse, Rat brain tissue lysates. IHC-P: FFPE human kidney renal cell carcinoma tissue sections.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)
    Western blot - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)
    All lanes : HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466)
    Lane 5 : Brain (Mouse) Tissue Lysate
    Lane 6 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa
    Observed band size: 36 kDa


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab201822 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)

    IHC image of GAPDH staining in a section of formalin-fixed paraffin-embedded human kidney renal cell carcinoma tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab201822 at 1/100 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)
    HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)

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