Alexa Fluor® 488 Anti-GAPDH antibody [EPR16884] (ab204276)
![Alexa Fluor® 488 Anti-GAPDH antibody [EPR16884] (ab204276)](https://www.abcam.com/ps/products/204/ab204276/Images/ab204276-248143-anti-gapdh-antibody-epr16884-alexa-fluor-488-immunofluorescence.jpg "Alexa Fluor® 488 Anti-GAPDH antibody [EPR16884] (ab204276)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR16884] to GAPDH
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Overview
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Product name
Alexa Fluor® 488 Anti-GAPDH antibody [EPR16884]
See all GAPDH primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [EPR16884] to GAPDH -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Recombinant fragment within Mouse GAPDH aa 1-150. The exact sequence is proprietary.
Database link: P16858 -
Positive control
- ICC/IF: HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16884 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GAPDH antibody [EPR16884] (ab204276)
ab204276 staining GAPDH in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204276 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Alexa Fluor® 488 Anti-GAPDH antibody [EPR16884] (ab204276)](https://www.abcam.com/ps/products/204/ab204276/Images/ab204276-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 488 Anti-GAPDH antibody [EPR16884] (ab204276)
