Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Wednesday March 17, 2021

Anti-ICOS Ligand/ICOSL antibody [EPR20786] (ab230946)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20786] to ICOS Ligand/ICOSL
Suitable for: WB, IP
Reacts with: Human

Product name

Anti-ICOS Ligand/ICOSL antibody [EPR20786]
See all ICOS Ligand/ICOSL primary antibodies
Description

Rabbit monoclonal [EPR20786] to ICOS Ligand/ICOSL
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: His-tagged recombinant human ICOS Ligand/ICOSL protein (aa19-258); Untreated HUVEC whole cell lysate, HUVEC whole cell lysate treated with TNF-alpha; HuT-78 and JAR whole cell lysates. IP: JAR whole cell lysate.
General notes
This product was previously labelled as ICOS Ligand

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20786
Isotype

IgG
Research areas

Immunoprecipitation - Anti-ICOS Ligand/ICOSL antibody [EPR20786] (ab230946)

ICOS Ligand/ICOSL was immunoprecipitated from 0.35 mg JAR (human placenta choriocarcinoma cell line) whole cell lysate with ab230946 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230946 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: JAR (human placenta choriocarcinoma cell line) whole cell lysate 10 µg (Input).
Lane 2: ab230946 IP in JAR whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230946 in JAR whole cell lysate (-).

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20786] (ab230946)

All lanes : Anti-ICOS Ligand/ICOSL antibody [EPR20786] (ab230946) at 1/1000 dilution

Lane 1 : HuT-78 (human Sezary syndrome cutaneous T lymphocyte) whole cell lysate
Lane 2 : JAR (human placenta choriocarcinoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 33 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer: 5% NFDM/TBST.

Exposure times:

Lane 1: 15 seconds.

Lane 2: 84 seconds.

ICOS ligand/ICOSL is highly glycosylated (PMID11983910).
Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20786] (ab230946)

All lanes : Anti-ICOS Ligand/ICOSL antibody [EPR20786] (ab230946) at 1/1000 dilution

Lane 1 : Untreated HUVEC (human umbilical vein endothelial cell line) whole cell lysate
Lane 2 : HUVEC whole cell lysate treated with tumor necrosis factor (TNF-alpha, ab9642) 200 unit/ml for 24 hours

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 33 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer: 5% NFDM/TBST.

Exposure time: 58 seconds.

The level of ICOS Ligand/ICOSL expression can be elevated by TNF-alpha treatment (PMID 11983910,PMID16049332).
Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20786] (ab230946)

Anti-ICOS Ligand/ICOSL antibody [EPR20786] (ab230946) at 1/1000 dilution + His-tagged recombinant human ICOS ligand/ICOSL protein (aa19-258) at 0.01 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 33 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 15 seconds.
Anti-ICOS Ligand/ICOSL antibody [EPR20786] (ab230946)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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