Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ICOS Ligand/ICOSL antibody [EPR20786] - BSA and Azide free (ab239524)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20786] to ICOS Ligand/ICOSL - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-ICOS Ligand/ICOSL antibody [EPR20786] - BSA and Azide free
    See all ICOS Ligand/ICOSL primary antibodies

  • Description

    Rabbit monoclonal [EPR20786] to ICOS Ligand/ICOSL - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Untreated HUVEC whole cell lysate and HUVEC whole cell lysate treated with TNF-alpha.

  • General notes

    ab239524 is the carrier-free version of ab230946 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab239524 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as ICOS Ligand

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20786

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-ICOS Ligand/ICOSL antibody [EPR20786] - BSA and Azide free (ab239524)
    Immunoprecipitation - Anti-ICOS Ligand/ICOSL antibody [EPR20786] - BSA and Azide free (ab239524)

    ICOS Ligand/ICOSL was immunoprecipitated from 0.35 mg JAR (human placenta choriocarcinoma cell line) whole cell lysate with ab230946 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230946 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: JAR (human placenta choriocarcinoma cell line) whole cell lysate 10 µg (Input).
    Lane 2: ab230946 IP in JAR whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230946 in JAR whole cell lysate (-).

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230946).

  • Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20786] - BSA and Azide free (ab239524)
    Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20786] - BSA and Azide free (ab239524)
    All lanes : Anti-ICOS Ligand/ICOSL antibody [EPR20786] (ab230946) at 1/1000 dilution

    Lane 1 : Untreated HUVEC (human umbilical vein endothelial cell line) whole cell lysate
    Lane 2 : HUVEC whole cell lysate treated with tumor necrosis factor (TNF-alpha, ab9642) 200 unit/ml for 24 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 58 seconds.

    The level of ICOS Ligand/ICOSL expression can be elevated by TNF-alpha treatment (PMID 11983910,PMID16049332).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230946).

     

  • Anti-ICOS Ligand/ICOSL antibody [EPR20786] - BSA and Azide free (ab239524)
    Anti-ICOS Ligand/ICOSL antibody [EPR20786] - BSA and Azide free (ab239524)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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