Anti-ICOS Ligand/ICOSL antibody [EPR20786] - BSA and Azide free (ab239524)
![Anti-ICOS Ligand/ICOSL antibody [EPR20786] - BSA and Azide free (ab239524)](https://www.abcam.com/ps/products/239/ab239524/Images/ab239524-320711-anti-icos-ligand-antibody-epr20786-immunoprecipitation.jpg "Anti-ICOS Ligand/ICOSL antibody [EPR20786] - BSA and Azide free (ab239524)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20786] to ICOS Ligand/ICOSL - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-ICOS Ligand/ICOSL antibody [EPR20786] - BSA and Azide free
See all ICOS Ligand/ICOSL primary antibodies -
Description
Rabbit monoclonal [EPR20786] to ICOS Ligand/ICOSL - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Untreated HUVEC whole cell lysate and HUVEC whole cell lysate treated with TNF-alpha.
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General notes
ab239524 is the carrier-free version of ab230946 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab239524 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as ICOS Ligand
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20786 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-ICOS Ligand/ICOSL antibody [EPR20786] - BSA and Azide free (ab239524)
ICOS Ligand/ICOSL was immunoprecipitated from 0.35 mg JAR (human placenta choriocarcinoma cell line) whole cell lysate with ab230946 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230946 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: JAR (human placenta choriocarcinoma cell line) whole cell lysate 10 µg (Input).
Lane 2: ab230946 IP in JAR whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230946 in JAR whole cell lysate (-).Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230946).
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![Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20786] - BSA and Azide free (ab239524)](https://www.abcam.com/ps/products/239/ab239524/Images/ab239524-317950-anti-icos-ligand-antibody-epr20786-western-blot.jpg)
Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20786] - BSA and Azide free (ab239524)All lanes : Anti-ICOS Ligand/ICOSL antibody [EPR20786] (ab230946) at 1/1000 dilution
Lane 1 : Untreated HUVEC (human umbilical vein endothelial cell line) whole cell lysate
Lane 2 : HUVEC whole cell lysate treated with tumor necrosis factor (TNF-alpha, ab9642) 200 unit/ml for 24 hours
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 58 seconds.
The level of ICOS Ligand/ICOSL expression can be elevated by TNF-alpha treatment (PMID 11983910,PMID16049332).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230946).
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![Anti-ICOS Ligand/ICOSL antibody [EPR20786] - BSA and Azide free (ab239524)](https://www.abcam.com/ps/products/239/ab239524/Images/ab239524-3-benefits-of-recombinant-antibodies.png)
Anti-ICOS Ligand/ICOSL antibody [EPR20786] - BSA and Azide free (ab239524)
