Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µg 278 083 ₸

Order now and get it on Wednesday March 10, 2021

Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] (ab257321)

Key features and details

Mouse monoclonal [ICOSL/3111] to ICOS Ligand/ICOSL
Suitable for: Flow Cyt, IHC-P, ICC/IF
Reacts with: Human
Isotype: IgG1

Product name

Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111]
See all ICOS Ligand/ICOSL primary antibodies
Description

Mouse monoclonal [ICOSL/3111] to ICOS Ligand/ICOSL
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human

See all applications and species data

Immunogen

Recombinant fragment within Human ICOS Ligand/ICOSL aa 23-149. The exact sequence is proprietary.
Database link: O75144
Positive control
- IHC-P: Human prostate carcinoma and colon carcinoma tissues; Flow Cyt: U937 cells; ICC/IF: U937 cells.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from bioreactor concentrate.
Clonality

Monoclonal
Clone number

ICOSL/3111
Isotype

IgG1
Light chain type

kappa
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] (ab257321)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma staining ICOS Ligand/ICOSL with ab257321 at 2µg/ml for 30 minutes at room temperature.
Immunocytochemistry/ Immunofluorescence - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] (ab257321)

Immunocytochemistry/Immunofluorescent analysis of U937 cells staining ICOS ligand/ICOSL with ab257321 at 2µg/ml, followed by goat anti-Mouse IgG conjugated to CF488 secondary antibody (green)/ Nuclei counterstained with Reddot (red).
Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] (ab257321)

Flow Cytometric analysis of U937 cells staining ICOSL with ab257321, followed by a goat anti-Mouse IgG-CF488 secondary antibody (blue). Isotype control (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] (ab257321)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma staining ICOS Ligand/ICOSL with ab257321 at 2µg/ml for 30 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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