Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] (ab257321)

Key features and details

  • Mouse monoclonal [ICOSL/3111] to ICOS Ligand/ICOSL
  • Suitable for: Flow Cyt, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111]
    See all ICOS Ligand/ICOSL primary antibodies

  • Description

    Mouse monoclonal [ICOSL/3111] to ICOS Ligand/ICOSL

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human ICOS Ligand/ICOSL aa 23-149. The exact sequence is proprietary.
    Database link: O75144

  • Positive control

    • IHC-P: Human prostate carcinoma and colon carcinoma tissues; Flow Cyt: U937 cells; ICC/IF: U937 cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    Purified from bioreactor concentrate.

  • Clonality

    Monoclonal

  • Clone number

    ICOSL/3111

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] (ab257321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] (ab257321)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma staining ICOS Ligand/ICOSL with ab257321 at 2µg/ml for 30 minutes at room temperature.

  • Immunocytochemistry/ Immunofluorescence - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] (ab257321)
    Immunocytochemistry/ Immunofluorescence - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] (ab257321)

    Immunocytochemistry/Immunofluorescent analysis of U937 cells staining ICOS ligand/ICOSL with ab257321 at 2µg/ml, followed by goat anti-Mouse IgG conjugated to CF488 secondary antibody (green)/ Nuclei counterstained with Reddot (red).

  • Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] (ab257321)
    Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] (ab257321)

    Flow Cytometric analysis of U937 cells staining ICOSL with ab257321, followed by a goat anti-Mouse IgG-CF488 secondary antibody (blue). Isotype control (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] (ab257321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] (ab257321)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma staining ICOS Ligand/ICOSL with ab257321 at 2µg/ml for 30 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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