Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ICOS Ligand/ICOSL antibody [EPR20240] - BSA and Azide free (ab251502)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20240] to ICOS Ligand/ICOSL - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-ICOS Ligand/ICOSL antibody [EPR20240] - BSA and Azide free
    See all ICOS Ligand/ICOSL primary antibodies

  • Description

    Rabbit monoclonal [EPR20240] to ICOS Ligand/ICOSL - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251502 is the carrier-free version of ab209262 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251502 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as ICOS Ligand

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20240

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] - BSA and Azide free (ab251502)
    Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] - BSA and Azide free (ab251502)
    Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262) at 1/20000 dilution + His-tagged recombinant human ICOS ligand/ICOSL protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    This data was developed using ab209262, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] - BSA and Azide free (ab251502)
    Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] - BSA and Azide free (ab251502)
    All lanes : Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262) at 1/1000 dilution

    Lane 1 : Untreated JAR (Human placenta choriocarcinoma cell line) whole cell lysate
    Lane 2 : JAR whole cell lysate treated with PNGase F

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa


    Exposure time: 30 seconds


    This data was developed using ab209262, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    ICOS ligand/ICOSL is highly glycosylated (PMID 11983910).

    Observed band sizes: 60 kDa (glycosylated form); 35 kDa (non-glycosylated form).

  • Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] - BSA and Azide free (ab251502)
    Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] - BSA and Azide free (ab251502)
    All lanes : Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262) at 1/1000 dilution

    Lane 1 : Untreated HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
    Lane 2 : HUVEC whole cell lysate treated with tumor necrosis factor-a (TNF-alpha, ab9642) 200 unit/ml for 24 hours

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This data was developed using ab209262, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The level of ICOS Ligand/ICOSL expression can be elevated by TNF-alpha treatment (PMID: 11983910).

  • Immunoprecipitation - Anti-ICOS Ligand/ICOSL antibody [EPR20240] - BSA and Azide free (ab251502)
    Immunoprecipitation - Anti-ICOS Ligand/ICOSL antibody [EPR20240] - BSA and Azide free (ab251502)

    This data was developed using ab209262, the same antibody clone in a different buffer formulation.

    ICOS Ligand/ICOSL was immunoprecipitated from 0.35 mg of JAR (Human placenta choriocarcinoma cell line) whole cell lysate with ab209262 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab209262 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: JAR whole cell lysate, 10 µg (Input).

    Lane 2: ab209262 IP in JAR whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209262 in JAR whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-ICOS Ligand/ICOSL antibody [EPR20240] - BSA and Azide free (ab251502)
    Anti-ICOS Ligand/ICOSL antibody [EPR20240] - BSA and Azide free (ab251502)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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