Antibodies, Proteins, Kits and Reagents for Life Science

526 012 ₸ Product size

100 µg 526 012 ₸ 1 mg 4 690 ₸

Order now and get it on Tuesday March 09, 2021

Anti-ICOS Ligand/ICOSL antibody [EPR20240] - BSA and Azide free (ab251502)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20240] to ICOS Ligand/ICOSL - BSA and Azide free
Suitable for: WB, IP
Reacts with: Human

Product name

Anti-ICOS Ligand/ICOSL antibody [EPR20240] - BSA and Azide free
See all ICOS Ligand/ICOSL primary antibodies
Description

Rabbit monoclonal [EPR20240] to ICOS Ligand/ICOSL - BSA and Azide free
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
General notes
ab251502 is the carrier-free version of ab209262 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab251502 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

This product was previously labelled as ICOS Ligand
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20240
Isotype

IgG
Research areas

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] - BSA and Azide free (ab251502)

Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262) at 1/20000 dilution + His-tagged recombinant human ICOS ligand/ICOSL protein at 0.01 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 33 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?

Exposure time: 10 seconds

This data was developed using ab209262, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] - BSA and Azide free (ab251502)

All lanes : Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262) at 1/1000 dilution

Lane 1 : Untreated JAR (Human placenta choriocarcinoma cell line) whole cell lysate
Lane 2 : JAR whole cell lysate treated with PNGase F

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 33 kDa

Exposure time: 30 seconds

This data was developed using ab209262, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

ICOS ligand/ICOSL is highly glycosylated (PMID 11983910).

Observed band sizes: 60 kDa (glycosylated form); 35 kDa (non-glycosylated form).
Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] - BSA and Azide free (ab251502)

All lanes : Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262) at 1/1000 dilution

Lane 1 : Untreated HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lane 2 : HUVEC whole cell lysate treated with tumor necrosis factor-a (TNF-alpha, ab9642) 200 unit/ml for 24 hours

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 33 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

This data was developed using ab209262, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

The level of ICOS Ligand/ICOSL expression can be elevated by TNF-alpha treatment (PMID: 11983910).
Immunoprecipitation - Anti-ICOS Ligand/ICOSL antibody [EPR20240] - BSA and Azide free (ab251502)

This data was developed using ab209262, the same antibody clone in a different buffer formulation.

ICOS Ligand/ICOSL was immunoprecipitated from 0.35 mg of JAR (Human placenta choriocarcinoma cell line) whole cell lysate with ab209262 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab209262 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: JAR whole cell lysate, 10 µg (Input).

Lane 2: ab209262 IP in JAR whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209262 in JAR whole cell lysate.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-ICOS Ligand/ICOSL antibody [EPR20240] - BSA and Azide free (ab251502)

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