Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD19 antibody [BU12] - BSA and Azide free (ab255925)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [BU12] to CD19 - BSA and Azide free
  • Suitable for: Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD19 antibody [BU12] - BSA and Azide free
    See all CD19 primary antibodies

  • Description

    Mouse monoclonal [BU12] to CD19 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus within Human CD19. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Flow Cyt: Human peripheral blood mononuclear cell.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255925 is the carrier-free version of ab254170. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Flow Cytometry - Anti-CD19 antibody [BU12] - BSA and Azide free (ab255925)
    Flow Cytometry - Anti-CD19 antibody [BU12] - BSA and Azide free (ab255925)

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CD19 with ab254170 at 1.07 µg/mL / Right compared with a Mouse monoclonal IgG / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody. Cells were stained with mouse IgG (Left) or ab254170 (Right). Then stained with anti-CD3 conjugated to Alexa Fluor® 647. Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254170).

  • Anti-CD19 antibody [BU12] - BSA and Azide free (ab255925)
    Anti-CD19 antibody [BU12] - BSA and Azide free (ab255925)

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