Anti-AP-A antibody (ab36122)
Key features and details
- Goat polyclonal to AP-A
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AP-A antibody
See all AP-A primary antibodies -
Description
Goat polyclonal to AP-A -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Dog, Pig
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Immunogen
Synthetic peptide corresponding to Human AP-A (internal sequence).
Sequence:EQYQKTSLAQEKEK
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Positive control
- Human testis lysate. Human kidney tissue sections.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AP-A antibody (ab36122)Anti-AP-A antibody (ab36122) at 1 µg/ml + Human Testis Lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 109 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Primary incubated for 1 hour. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody (ab36122)ab36122 at 3.8µg/ml staining AP-A in human kidney tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed, AP-staining. Image shows staining of basement membrane and the mesangium in the glomerulus.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-A antibody (ab36122) Image courtesy of Dr Carl Atkinson by Abreview.ab36122 staining AP-A in rat kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate. Samples were then blocked using 20% serum for 1 hour at 25°C and then incubated with ab36122 at 3µg/ml for 1 hour. The secondary used was an undiluted HRP conjugated polyclonal.
