Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (ab240298)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18118] to GATA3 (phospho S308) - BSA and Azide free
  • Suitable for: WB, IP, Dot blot, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free
    See all GATA3 primary antibodies

  • Description

    Rabbit monoclonal [EPR18118] to GATA3 (phospho S308) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Dot blot, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab240298 is the carrier-free version of ab186371. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240298 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Dot Blot - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (ab240298)
    Dot Blot - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (ab240298)

    Dot blot analysis of GATA3 (phospho S308) peptide (Lane 1) and non-phospho peptide (Lane 2) labeled using ab186371 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

    Blocking/Dilution buffer: 5% NFDM/TBST..

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186371).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (ab240298)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (ab240298)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling GATA3 (phospho S308) with ab186371 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on trophoblast cells of Human placenta is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186371).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (ab240298)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (ab240298)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma tissue labeling GATA3 (phospho S308) with ab186371 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on tumor cells of Human transitional cell carcinoma of bladder is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186371).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (ab240298)
    Immunoprecipitation - Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (ab240298)

    GATA3 (phospho S308) was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) cells treated with 1mM 8-Bromo-cAMP for 6 hours with ab186371 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab186371 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.

    Lane 1: Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours, 10 µg (Input).

    Lane 2: ab186371 IP in Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab186371 in Jurkat whole cell lysate treated with 1mM 8-Bromo-cAMP for 6 hours.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186371).

  • Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (ab240298)
    Anti-GATA3 (phospho S308) antibody [EPR18118] - BSA and Azide free (ab240298)

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