Anti-GATA3 antibody [mAbcam77073] (ab77073)
![Anti-GATA3 antibody [mAbcam77073] (ab77073)](https://www.abcam.com/ps/products/77/ab77073/Images/ab77073-92733-anti-gata3-antibody-mabcam77073-western-blot.jpg "Anti-GATA3 antibody [mAbcam77073] (ab77073)")
Key features and details
- Mouse monoclonal [mAbcam77073] to GATA3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GATA3 antibody [mAbcam77073]
See all GATA3 primary antibodies -
Description
Mouse monoclonal [mAbcam77073] to GATA3 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Pig
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following human tissue lysates: Thymus; Lymph Node; Spleen; Tonsil.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
mAbcam77073 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GATA3 antibody [mAbcam77073] (ab77073)All lanes : Anti-GATA3 antibody [mAbcam77073] (ab77073) at 5 µg/ml
Lane 1 : Human thymus tissue lysate - total protein (ab30146)
Lane 2 : Human lymph node tissue lysate - total protein (ab29871)
Lane 3 : Human spleen tissue lysate - total protein (ab29699)
Lane 4 : Tonsil (Human) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
The binding pattern of ab77073 in human thymus, lymph node, spleen and tonsil tissue lysates is similar to that of the polyclonal antibody to the same target (ab32858) in MOLT4, Jurkat, THP1 and Ramos whole cell lysates.
