Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)
![Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)](https://www.abcam.com/ps/products/270/ab270750/Images/ab270750-6-anti-sec23asec23b-antibody-epr22723102-immunohistochemistry-rat-kidney.jpg "Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22723-102] to SEC23B + SEC23A
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Overview
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Product name
Anti-SEC23B + SEC23A antibody [EPR22723-102] -
Description
Rabbit monoclonal [EPR22723-102] to SEC23B + SEC23A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatHumanWB MouseRatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Purified LIF/His-tagged SEC23B recombinant protein, His-tagged SEC23A recombinant protein; human liver lysate; mouse liver lysate; MEF, HeLa, Raji, C6, RAW 264.7, PC-12, and NIH/3T3 whole cell lysates. IHC-P: Human cerebrum tissue; mouse liver tissue, rat kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22723-102 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling SEC23B + SEC23A with ab270750 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive cytoplasmic staining on rat kidney (PMID: 22745161).
The section was incubated with ab270750 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)](https://www.abcam.com/ps/products/270/ab270750/Images/ab270750-383460-anti-sec23asec23b-antibody-epr22723102-western-blot-mef-hela-raji-mouse-liver-c6-raw-2647pc12-nih3t3-lysates.jpg)
Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)All lanes : Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750) at 1/1000 dilution
Lane 1 : MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Raji (human burkitts lymphoma b lymphocyte) whole cell lysate
Lane 4 : Mouse liver tissue lysate
Lane 5 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 6 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 7 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 8 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-7 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Lane 8 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 mg/ml
Predicted band size: 86 kDa
Observed band size: 86 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 22745161).
Exposure time: Lanes 1-4: 26 seconds;Lanes 5-8: 10 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)](https://www.abcam.com/ps/products/270/ab270750/Images/ab270750-5-anti-sec23asec23b-antibody-epr22723102-immunohistochemistry-mouse-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling SEC23B + SEC23A with ab270750 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive cytoplasmic staining on mouse liver (PMID: 22745161).
The section was incubated with ab270750 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)](https://www.abcam.com/ps/products/270/ab270750/Images/ab270750-383459-anti-sec23asec23b-antibody-epr22723102-western-blot-human-liver-lysate.jpg)
Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750) at 1/1000 dilution + Human liver tissue lysate at 20 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 86 kDa
Observed band size: 86 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 22745161).
Exposure time: 3 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)](https://www.abcam.com/ps/products/270/ab270750/Images/ab270750-4-anti-sec23asec23b-antibody-epr22723102-immunohistochemistry-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling SEC23B + SEC23A with ab270750 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive cytoplasmic staining on human cerebrum (PMID: 22745161) is observed.
The section was incubated with ab270750 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)](https://www.abcam.com/ps/products/270/ab270750/Images/ab270750-383458-anti-sec23asec23b-antibody-epr22723102-western-blot-purified-lifhistagged-sec23b-recombinant-protein-histagged-sec23a-recombinant-protein-lysates.jpg)
Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)All lanes : Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750) at 1/1000 dilution
Lane 1 : Purified LIF/His-tagged SEC23B recombinant protein (aa127-392), 10 ng
Lane 2 : His-tagged SEC23A recombinant protein (aa127-392)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 86 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Both the recombinant proteins were made in house.
SEC23B recombinant protein was expressed from a mammalian - HEK-293 expression system. The LIF tag is about 40 kDa.
SEC23A recombinant protein was from E.coil extracts.
Exposure time: 3 seconds.
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![Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)](https://www.abcam.com/ps/products/270/ab270750/Images/ab270750-7-benefits-of-recombinant-antibodies.png)
Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)