Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (ab232655)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12294-85] to RAB11-FIP2 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free
    See all RAB11-FIP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12294-85] to RAB11-FIP2 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon cancer tissue.

  • General notes

    ab232655 is the carrier-free version of ab180504.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12294-85

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (ab232655)
    Flow Cytometry - Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (ab232655)

    Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling RAB11-FIP2 with purified ab180504 at 1:50 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180504).

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (ab232655)
    Immunocytochemistry/ Immunofluorescence - Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (ab232655)

    Immunocytochemistry/ Immunofluorescence analysis of HEK-293 (human embryonic kidney epithelial cell) cells labeling RAB11-FIP2 with purified ab180504 at 1:100 (5.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180504).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (ab232655)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (ab232655)

    Immunohistochemical analysis of paraffin-embedded, Human brain tissue labeling RAB11-FIP2 with unpurified 
    ab180504 at a 1/250 dilution. Counter stained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180504).

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (ab232655)
    Immunocytochemistry/ Immunofluorescence - Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (ab232655)

    Immunofluorescence analysis of, paraformaldehyde-fixed, 293 cells labeling RAB11-FIP2 with unpurified 
    ab180504 at a 1/100 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®555) was used at a 1/250. In blue DAPI staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180504).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (ab232655)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (ab232655)

    Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling RAB11-FIP2 with Purified ab180504 at 1:500 dilution (1.09 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180504).

  • Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (ab232655)
    Anti-RAB11-FIP2 antibody [EPR12294-85] - BSA and Azide free (ab232655)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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