Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12294-85] to RAB11-FIP2
Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-RAB11-FIP2 antibody [EPR12294-85]
See all RAB11-FIP2 primary antibodies
Description

Rabbit monoclonal [EPR12294-85] to RAB11-FIP2
Host species

Rabbit
Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- 293 and HeLa whole cell lysate (ab150035); Human brain tissue; 293 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12294-85
Isotype

IgG
Research areas

Western blot - Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)

All lanes : Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504) at 1/10000 dilution

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : Rat brain lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 58 kDa
Observed band size: 58 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling RAB11-FIP2 with Purified ab180504 at 1:500 dilution (1.09 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.
Immunocytochemistry/ Immunofluorescence - Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)

Immunocytochemistry/ Immunofluorescence analysis of HEK-293 (human embryonic kidney epithelial cell) cells labeling RAB11-FIP2 with purified ab180504 at 1:100 (5.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry - Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)

Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling RAB11-FIP2 with purified ab180504 at 1:50 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)

All lanes : Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504) at 1/10000 dilution (unpurified)

Lane 1 : 293 cell lysate
Lane 2 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 58 kDa
Observed band size: 58 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)

Immunohistochemical analysis of paraffin-embedded, Human brain tissue labeling RAB11-FIP2 with unpurified
ab180504 at a 1/250 dilution. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)

Immunofluorescence analysis of, paraformaldehyde-fixed, 293 cells labeling RAB11-FIP2 with unpurified
ab180504 at a 1/100 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®555) was used at a 1/250. In blue DAPI staining.
Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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