Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12294-85] to RAB11-FIP2
  • Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RAB11-FIP2 antibody [EPR12294-85]
    See all RAB11-FIP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12294-85] to RAB11-FIP2

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293 and HeLa whole cell lysate (ab150035); Human brain tissue; 293 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12294-85

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)
    Western blot - Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)
    All lanes : Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504) at 1/10000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 3 : Mouse brain lysates
    Lane 4 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 58 kDa
    Observed band size: 58 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling RAB11-FIP2 with Purified ab180504 at 1:500 dilution (1.09 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)
    Immunocytochemistry/ Immunofluorescence - Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)

    Immunocytochemistry/ Immunofluorescence analysis of HEK-293 (human embryonic kidney epithelial cell) cells labeling RAB11-FIP2 with purified ab180504 at 1:100 (5.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)
    Flow Cytometry - Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)

    Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling RAB11-FIP2 with purified ab180504 at 1:50 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)
    Western blot - Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)
    All lanes : Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504) at 1/10000 dilution (unpurified)

    Lane 1 : 293 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 58 kDa
    Observed band size: 58 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)

    Immunohistochemical analysis of paraffin-embedded, Human brain tissue labeling RAB11-FIP2 with unpurified 
    ab180504 at a 1/250 dilution. Counter stained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)
    Immunocytochemistry/ Immunofluorescence - Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)

    Immunofluorescence analysis of, paraformaldehyde-fixed, 293 cells labeling RAB11-FIP2 with unpurified 
    ab180504 at a 1/100 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®555) was used at a 1/250. In blue DAPI staining.

  • Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)
    Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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