Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] - BSA and Azide free (ab251484)
![Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] - BSA and Azide free (ab251484)](https://www.abcam.com/ps/products/251/ab251484/Images/ab251484-408310-anti-nucleophosmin-citrulline-r196-antibody-epr20172-bsa-and-azide-free-western-blot.jpg "Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] - BSA and Azide free (ab251484)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20172] to Nucleophosmin (citrulline R196) - BSA and Azide free
- Suitable for: Dot blot, WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] - BSA and Azide free
See all Nucleophosmin primary antibodies -
Description
Rabbit monoclonal [EPR20172] to Nucleophosmin (citrulline R196) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251484 is the carrier-free version of ab208015. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251484 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR20172 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] - BSA and Azide free (ab251484)All lanes : Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] (ab208015) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) transfected with a control vector containing GFP tag, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
Lane 2 : NIH/3T3 transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsThis data was developed using ab208015, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
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![Western blot - Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] - BSA and Azide free (ab251484)](https://www.abcam.com/ps/products/251/ab251484/Images/ab251484-406164-anti-nucleophosmin-citrulline-r196-antibody-epr20172-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] - BSA and Azide free (ab251484)All lanes : Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] (ab208015) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a control vector containing GFP tag, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
Lane 2 : HEK-293T transfected with GFP-tagged PADI2 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
Lane 3 : C6 (rat glial tumor cell line) transfected with a control vector containing GFP tag, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
Lane 4 : C6 transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?This data was developed using ab208015, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-2: 5 seconds; Lanes 3-4: 3 minutes.
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![Immunoprecipitation - Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] - BSA and Azide free (ab251484)](https://www.abcam.com/ps/products/251/ab251484/Images/ab251484-2-anti-nucleophosmin-citrulline-r196-antibody-epr20172-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] - BSA and Azide free (ab251484)This data was developed using ab208015, the same antibody clone in a different buffer formulation.Nucleophosmin (citrulline R196) was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged PADI4 expression vector for 24h then treated with 10 mM CaCl2 and 10 µM ionomycin for 2h, whole cell lysate with ab208015 at 1/30 dilution. Western blot was perfromed from the immunoprecipitate using ab208015 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/10000 dilution. Lane 1: HEK-293T transfected with GFP-tagged PADI4 expression vector for 24h then treated with 10 mM CaCl2 and 10 µM ionomycin for 2h, whole cell lysate 10μg (Input). Lane 2: ab208015 IP in HEK-293T transfected with GFP-tagged PADI4 expression vector for 24h then treated with 10 mM CaCl2 and 10 µM ionomycin for 2h, whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208015 in HEK-293T transfected with GFP-tagged PADI4 expression vector for 24h then treated with 10mM CaCl2 and 10 µM ionomycin for 2h, whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST Exposure time : lesss than 1 second. -
![Dot Blot - Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] - BSA and Azide free (ab251484)](https://www.abcam.com/ps/products/251/ab251484/Images/ab251484-1-anti-nucleophosmin-citrulline-r196-antibody-epr20172-bsa-and-azide-free-dot-blot.jpg)
Dot Blot - Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] - BSA and Azide free (ab251484)This data was developed using ab208015, the same antibody clone in a different buffer formulation.Dot blot analysis of Nucleophosmin (citruline R196) labeled with ab208015 at 1/1000 dilution. Lane 1: Nucleophosmin (citrulline R196) peptide. Lane 2: Nucleophosmin non-citrulline peptide. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody. Blocking/Dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes. -
![Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] - BSA and Azide free (ab251484)](https://www.abcam.com/ps/products/251/ab251484/Images/ab251484-5-benefits-of-recombinant-antibodies.png)
Anti-Nucleophosmin (citrulline R196) antibody [EPR20172] - BSA and Azide free (ab251484)