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Signal Transduction Protein Trafficking Vesicle Transport Regulation

Human ZFYVE16 (Endofin) knockout HCT116 cell lysate (ab259248)

Human ZFYVE16 (Endofin) knockout HCT116 cell lysate (ab259248)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

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Overview

  • Product name

    Human ZFYVE16 (Endofin) knockout HCT116 cell lysate
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HCT116
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon3.
  • Passage number

  • Knockout validation

    Sanger Sequencing
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab255555 - Human wild-type HCT116 cell lysate 1 x 100µg
    ab263736 - Human ZFYVE16 knockout HCT116 cell lysate 1 x 100µg
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
  • Cell type

    epithelial
  • Disease

    Carcinoma
  • STR Analysis

    Amelogenin X D5S818: 10, 11 D13S317: 10, 12 D7S820: 11, 12 D16S539: 11, 13 vWA: 17, 22 TH01: 8,9 TPOX: 8, 9 CSF1PO: 7, 10

Target

  • Function

    May be involved in regulating membrane trafficking in the endosomal pathway. Overexpression induces endosome aggregation. Required to target TOM1 to endosomes.
  • Tissue specificity

    Widely expressed. Highly expressed in kidney, placenta and lung. Expressed at intermediate level in heart, brain, skeletal muscle, spleen and liver. Weakly expressed in colon, thymus and peripheral blood lymphocytes.
  • Sequence similarities

    Contains 1 FYVE-type zinc finger.
  • Domain

    The FYVE-type zinc finger is necessary and sufficient for its localization into early endosomes and mediates the association with PI3P.
  • Cellular localization

    Cytoplasm. Early endosome membrane. Localized to early endosomes. Membrane-associated, probably via its association with phosphatidylinositol 3-phosphate.
  • Target information above from: UniProt accession Q7Z3T8 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • AI035632
    • B130024H06Rik
    • B130031L15
    • DKFZp686E13162
    • Endofin
    • Endosomal associated FYVE domain protein
    • Endosome associated FYVE domain protein
    • Endosome-associated FYVE domain protein
    • KIAA0305
    • KIAA0305;
    • mKIAA0305
    • OTTMUSP00000029589
    • RGD1564784
    • ZFY16_HUMAN
    • ZFYVE16
    • Zinc finger FYVE domain containing protein 16
    • Zinc finger FYVE domain-containing protein 16
    • Zinc finger, FYVE domain containing 16
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab255555 - Human wild-type HCT116 cell lysate 1 x 100µg
    ab263736 - Human ZFYVE16 knockout HCT116 cell lysate 1 x 100µg
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
  • Cell type

    epithelial
  • Disease

    Carcinoma
  • STR Analysis

    Amelogenin X D5S818: 10, 11 D13S317: 10, 12 D7S820: 11, 12 D16S539: 11, 13 vWA: 17, 22 TH01: 8,9 TPOX: 8, 9 CSF1PO: 7, 10

Images

  • Sanger Sequencing - Human ZFYVE16 knockout HCT116 cell lysate (ab259248)
    Sanger Sequencing - Human ZFYVE16 knockout HCT116 cell lysate (ab259248)
    Homozygous: 2 bp deletion in exon3

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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