Anti-VPS35 antibody - C-terminal (ab226180)
Key features and details
- Rabbit polyclonal to VPS35 - C-terminal
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-VPS35 antibody - C-terminal
See all VPS35 primary antibodies -
Description
Rabbit polyclonal to VPS35 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Sheep, Rabbit, Horse, Cow, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
-
Immunogen
Synthetic peptide within Human VPS35 aa 746-796 (C terminal). The exact sequence is proprietary. (NP_060676.2).
Database link: Q96QK1 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-I and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226180 was affinity purified using an epitope specific to VPS35 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-VPS35 antibody - C-terminal (ab226180)All lanes : Anti-VPS35 antibody - C-terminal (ab226180) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 92 kDa
Exposure time: 30 seconds
-
Immunoprecipitation - Anti-VPS35 antibody - C-terminal (ab226180)VPS35 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226180 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226180 at 0.4 µg/ml.
Lane 1: ab226180 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
