HRP Anti-VPS35 antibody [EPR11501(B)] (ab215340)
![HRP Anti-VPS35 antibody [EPR11501(B)] (ab215340)](https://www.abcam.com/ps/products/215/ab215340/Images/ab215340-310954-ab215340-KOHRPHDRE.jpg "HRP Anti-VPS35 antibody [EPR11501(B)] (ab215340)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR11501(B)] to VPS35
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
- Conjugation: HRP
Overview
-
Product name
HRP Anti-VPS35 antibody [EPR11501(B)]
See all VPS35 primary antibodies -
Description
HRP Rabbit monoclonal [EPR11501(B)] to VPS35 -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HepG2, A549, MOLT4, NIH3T3, Hap1 WT (shows pos.) and Hap1 VPS35 KO (shows neg.)
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11501(B) -
Isotype
IgG -
Research areas
Images
-
Western blot - HRP Anti-VPS35 antibody [EPR11501(B)] (ab215340)All lanes : HRP Anti-VPS35 antibody [EPR11501(B)] (ab215340) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : VPS35 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 92 kDa
Exposure time: 1 minuteab215340 was shown to recognize VPS35 in wild-type HAP1 cells as signal was lost at the expected MW in VPS35 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and VPS35 knockout samples were subjected to SDS-PAGE. Ab215340 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
-
![Western blot - HRP Anti-VPS35 antibody [EPR11501(B)] (ab215340)](https://www.abcam.com/ps/products/215/ab215340/Images/ab215340-283926-WBab2153401.jpg)
Western blot - HRP Anti-VPS35 antibody [EPR11501(B)] (ab215340)All lanes : HRP Anti-VPS35 antibody [EPR11501(B)] (ab215340) at 1/5000 dilution
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg
Lane 3 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 5 : HAP1 WT at 20 µg
Lane 6 : VPS35 knockout HAP1 at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 92 kDa
Exposure time: 20 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab215340 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
-
![HRP Anti-VPS35 antibody [EPR11501(B)] (ab215340)](https://www.abcam.com/ps/products/215/ab215340/Images/ab215340-3-benefits-of-recombinant-antibodies.png)
HRP Anti-VPS35 antibody [EPR11501(B)] (ab215340)
