Anti-FSTL1/FRP antibody [EPR21933-155] - BSA and Azide free (ab234961)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21933-155] to FSTL1/FRP - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-FSTL1/FRP antibody [EPR21933-155] - BSA and Azide free
See all FSTL1/FRP primary antibodies -
Description
Rabbit monoclonal [EPR21933-155] to FSTL1/FRP - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat and mouse E14 embryo lysate; C6 cell lysate.
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General notes
ab234961 is the carrier-free version of ab223287 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab234961 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as FSTL1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21933-155 -
Isotype
IgG -
Research areas
Images
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FSTL1/FRP was immunoprecipitated from 0.35 mg rat embryo lysate with ab223287 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223287 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: Rat embryo lysate 10 μg (Input).
Lane 2: ab223287 IP in rat embryo lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223287 in rat embryo lysate (-).Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 minute.The molecular mass observed is consistent with what has been described in the literature (PMID: 28574994).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223287).
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All lanes : Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287) at 1/1000 dilution
Lane 1 : Rat E14 embryo lysate
Lane 2 : Mouse E14 embryo lysate
Lane 3 : C6 (Rat glial tumor cell line) cell lysate
Lane 4 : Rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 43-50 kDa why is the actual band size different from the predicted?
Exposure time: 48 secondsBlocking/diluting buffer and concentration: 5% NFDM/TBST.
The molecular mass observed is consistent with what has been described in the literature (PMID: 28574994).
Negative control: Rat liver (PMID: 20861081).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223287).
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