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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-FSTL1/FRP antibody [EPR21933-155] - BSA and Azide free (ab234961)

Anti-FSTL1/FRP antibody [EPR21933-155] - BSA and Azide free (ab234961)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21933-155] to FSTL1/FRP - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-FSTL1/FRP antibody [EPR21933-155] - BSA and Azide free
    See all FSTL1/FRP primary antibodies
  • Description

    Rabbit monoclonal [EPR21933-155] to FSTL1/FRP - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat and mouse E14 embryo lysate; C6 cell lysate.
  • General notes

    ab234961 is the carrier-free version of ab223287 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab234961 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as FSTL1

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21933-155
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Other
    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Cardiovascular
    • Atherosclerosis
    • Ischemia / Reperfusion

Images

  • Immunoprecipitation - Anti-FSTL1/FRP antibody [EPR21933-155] - BSA and Azide free (ab234961)
    Immunoprecipitation - Anti-FSTL1/FRP antibody [EPR21933-155] - BSA and Azide free (ab234961)

    FSTL1/FRP was immunoprecipitated from 0.35 mg rat embryo lysate with ab223287 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223287 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
    Lane 1: Rat embryo lysate 10 μg (Input).
    Lane 2: ab223287 IP in rat embryo lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223287 in rat embryo lysate (-).

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 minute.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 28574994).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223287).

  • Western blot - Anti-FSTL1/FRP antibody [EPR21933-155] - BSA and Azide free (ab234961)
    Western blot - Anti-FSTL1/FRP antibody [EPR21933-155] - BSA and Azide free (ab234961)
    All lanes : Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287) at 1/1000 dilution

    Lane 1 : Rat E14 embryo lysate
    Lane 2 : Mouse E14 embryo lysate
    Lane 3 : C6 (Rat glial tumor cell line) cell lysate
    Lane 4 : Rat liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa
    Observed band size: 43-50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 48 seconds


    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 28574994).

    Negative control: Rat liver (PMID: 20861081).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223287).

  • Anti-FSTL1/FRP antibody [EPR21933-155] - BSA and Azide free (ab234961)
    Anti-FSTL1/FRP antibody [EPR21933-155] - BSA and Azide free (ab234961)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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