Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287)
![Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287)](https://www.abcam.com/ps/products/223/ab223287/Images/ab223287-309865-anti-fstl1-antibody-epr21933-155-western-blot.jpg "Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21933-155] to FSTL1/FRP
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-FSTL1/FRP antibody [EPR21933-155]
See all FSTL1/FRP primary antibodies -
Description
Rabbit monoclonal [EPR21933-155] to FSTL1/FRP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP RatWB MouseRatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat and mouse E14 embryo lysate; C6 cell lysate; Human, mouse and rat serum. IP: Rat embryo lysate.
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General notes
This product was previously labelled as FSTL1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21933-155 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287)All lanes : Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287) at 1/1000 dilution
Lane 1 : Rat E14 embryo lysate
Lane 2 : Mouse E14 embryo lysate
Lane 3 : C6 (Rat glial tumor cell line) cell lysate
Lane 4 : Rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 43-45 kDa why is the actual band size different from the predicted?
Exposure time: 48 secondsBlocking/diluting buffer and concentration: 5% NFDM/TBST.
The molecular mass observed is consistent with what has been described in the literature (PMID: 28574994).
Negative control: Rat liver (PMID: 20861081).
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![Immunoprecipitation - Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287)](https://www.abcam.com/ps/products/223/ab223287/Images/ab223287-309867-anti-fstl1-antibody-epr21933-155-immunoprecipitation.jpg)
Immunoprecipitation - Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287)FSTL1/FRP was immunoprecipitated from 0.35 mg rat embryo lysate with ab223287 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223287 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: Rat embryo lysate 10 μg (Input).
Lane 2: ab223287 IP in rat embryo lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223287 in rat embryo lysate (-).Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 minute.The molecular mass observed is consistent with what has been described in the literature (PMID: 28574994).
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![Western blot - Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287)](https://www.abcam.com/ps/products/223/ab223287/Images/ab223287-309866-anti-fstl1-antibody-epr21933-155-western-blot.jpg)
Western blot - Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287)All lanes : Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287) at 1/1000 dilution
Lane 1 : Human serum
Lane 2 : Mouse serum
Lane 3 : Rat serum
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 40-50 kDa why is the actual band size different from the predicted?Blocking/diluting buffer and concentration: 5% NFDM/TBST.
Exposure times:
Lane 1 and 2: 3 minutes;
Lane 3: 15 seconds.The molecular mass observed is consistent with what has been described in the literature (PMID: 27234440).
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![Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287)](https://www.abcam.com/ps/products/223/ab223287/Images/ab223287-4-benefits-of-recombinant-antibodies.png)
Anti-FSTL1/FRP antibody [EPR21933-155] (ab223287)
