Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GRIM19 antibody [EPR4471(2)] (ab109017)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4471(2)] to GRIM19
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-GRIM19 antibody [EPR4471(2)]
    See all GRIM19 primary antibodies

  • Description

    Rabbit monoclonal [EPR4471(2)] to GRIM19

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, Ramos, Jurkat, and 293T cell lysates. IHC-P: Human kidney tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-GRIM19 antibody [EPR4471(2)] (ab109017)
    Western blot - Anti-GRIM19 antibody [EPR4471(2)] (ab109017)
    All lanes : Anti-GRIM19 antibody [EPR4471(2)] (ab109017) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : NDUFA13 knockout HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab109017 observed at 17 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab109017 Anti-GRIM19 antibody [EPR4471(2)] was shown to specifically react with GRIM19 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265863 (knockout cell lysate ab257136) was used.  Wild-type and GRIM19 knockout samples were subjected to SDS-PAGE.  ab109017 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRIM19 antibody [EPR4471(2)] (ab109017)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRIM19 antibody [EPR4471(2)] (ab109017)

    Immunohistochemical analysis of GRIM19 in paraffin-embedded Human kidney tissue using ab109017 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-GRIM19 antibody [EPR4471(2)] (ab109017)
    Western blot - Anti-GRIM19 antibody [EPR4471(2)] (ab109017)
    All lanes : Anti-GRIM19 antibody [EPR4471(2)] (ab109017) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Ramos cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 17 kDa

  • Anti-GRIM19 antibody [EPR4471(2)] (ab109017)
    Anti-GRIM19 antibody [EPR4471(2)] (ab109017)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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