Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GRIM19 antibody [6E1BH7] (ab110240)

Key features and details

  • Mouse monoclonal [6E1BH7] to GRIM19
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-GRIM19 antibody [6E1BH7]
    See all GRIM19 primary antibodies

  • Description

    Mouse monoclonal [6E1BH7] to GRIM19

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • WB: HeLa and Jurkat cell lysates; Human heart, Bovine heart, Rat heart, and Mouse heart isolated mitochondria. ICC/IF: Human fibroblasts. Flow Cyt: HeLa cells.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

     

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Images

  • Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240)
    Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240)
    All lanes : Anti-GRIM19 antibody [6E1BH7] (ab110240) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : NDUFA13 knockout HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab110240 observed at 17 kDa. Red - loading control ab181602 observed at 36 kDa. 

     ab110240 GRIM19 antibody [6E1BH7] was shown to specifically react with GRIM19 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265863 (knockout cell lysate ab257136) was used.  Wild-type and GRIM19 knockout samples were subjected to SDS-PAGE.  ab110240 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240)
    Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240)
    All lanes : Anti-GRIM19 antibody [6E1BH7] (ab110240) at 1 µg/ml

    Lane 1 : Human heart mitochondria
    Lane 2 : Bovine heart mitochondria
    Lane 3 : Rat heart mitochondria
    Lane 4 : Mouse heart mitochondria

    Predicted band size: 17 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-GRIM19 antibody [6E1BH7] (ab110240)
    Immunocytochemistry/ Immunofluorescence - Anti-GRIM19 antibody [6E1BH7] (ab110240)
    Mitochondrial localization of GRIM19 visualized by immunocytochemistry using ab110240 at a concentration of 1 µg/mL. Cultured Human fibroblasts were fixed, permeabilized and then labeled with ab110240 followed by Alexa® 488 goat-anti-mouse IgG.
  • Flow Cytometry - Anti-GRIM19 antibody [6E1BH7] (ab110240)
    Flow Cytometry - Anti-GRIM19 antibody [6E1BH7] (ab110240)
    HeLa cells were stained with 1 µg/mL ab110240 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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