Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µg 314 937 ₸

Order now and get it on Tuesday March 02, 2021

Anti-GRIM19 antibody [6E1BH7] (ab110240)

Key features and details

Mouse monoclonal [6E1BH7] to GRIM19
Suitable for: WB, ICC/IF, Flow Cyt
Knockout validated
Reacts with: Mouse, Rat, Cow, Human
Isotype: IgG2b

Product name

Anti-GRIM19 antibody [6E1BH7]
See all GRIM19 primary antibodies
Description

Mouse monoclonal [6E1BH7] to GRIM19
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is considered to be commercially sensitive.
Positive control
- WB: HeLa and Jurkat cell lysates; Human heart, Bovine heart, Rat heart, and Mouse heart isolated mitochondria. ICC/IF: Human fibroblasts. Flow Cyt: HeLa cells.
General notes

This antibody clone is manufactured by Abcam.

Product was previously marketed under the MitoSciences sub-brand.

If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline
Concentration information loading...
Purification notes

ab110240 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality

Monoclonal
Clone number

6E1BH7
Isotype

IgG2b
Light chain type

kappa
Research areas
- Cell Biology
- Apoptosis
- Nucleus
- Other
- Cancer
- Cell Death
- Apoptosis
- Nucleus
- Other

Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240)

All lanes : Anti-GRIM19 antibody [6E1BH7] (ab110240) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : NDUFA13 knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution

Predicted band size: 17 kDa
Observed band size: 17 kDa

Lanes 1-3: Merged signal (red and green). Green - ab110240 observed at 17 kDa. Red - loading control ab181602 observed at 36 kDa.

ab110240 GRIM19 antibody [6E1BH7] was shown to specifically react with GRIM19 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265863 (knockout cell lysate ab257136) was used. Wild-type and GRIM19 knockout samples were subjected to SDS-PAGE. ab110240 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-GRIM19 antibody [6E1BH7] (ab110240)

All lanes : Anti-GRIM19 antibody [6E1BH7] (ab110240) at 1 µg/ml

Lane 1 : Human heart mitochondria
Lane 2 : Bovine heart mitochondria
Lane 3 : Rat heart mitochondria
Lane 4 : Mouse heart mitochondria

Predicted band size: 17 kDa
Immunocytochemistry/ Immunofluorescence - Anti-GRIM19 antibody [6E1BH7] (ab110240)

Mitochondrial localization of GRIM19 visualized by immunocytochemistry using ab110240 at a concentration of 1 µg/mL. Cultured Human fibroblasts were fixed, permeabilized and then labeled with ab110240 followed by Alexa® 488 goat-anti-mouse IgG.
Flow Cytometry - Anti-GRIM19 antibody [6E1BH7] (ab110240)

HeLa cells were stained with 1 µg/mL ab110240 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com