Recombinant mouse CERK protein (Active) (ab271468)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 51% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant mouse CERK protein (Active)
See all CERK proteins and peptides -
Biological activity
Specific Activity: ≥10 pmole/min/μg
Assay Conditions: Assay conditions: CERK activity was measured at 37oC for 25 min in a buffer (20 mM HEPES pH7.2, 80 mM KCl, 3 mM CaCl2, 10 mM MgCl2, 2 mM DTT, 5 μM ATP) using a μ-Octylglucoside mixed micellar assay with 100 μM Ceramide substrate. ATP reduction was detected using a Luminescent Kinase Assay Platform.
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Purity
>= 51 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
MGAMGAAEPL HSVLWVKRRR CAVSLEPARA LLRWWRSPEP GPSAPGADAR SVLVSEIIAV EEKDDCEKHA SSGRWHKMEN PFAFTVHRVK RVRHHRWKWA RVTFWSADEQ LCHLWLQTLR GLLESLTSRP KHLLVFINPF GGKGQGKRIY EKTVAPLFTL ASITTEIIIT EHANQAKETL YEINTDSYDG IVCVGGDGMF SEVLHGVIGR TQQSAGIDPN HPRAVLVPST LRIGIIPAGS TDCVCYSTVG TNDAETSALH IIIGDSLAID VSSVHYHNTL LRYSVSLLGY GFYGDLIKDS EKKRWMGLVR YDFSGLKTFL SHQYYEGTLS FLPAQHTVGS PRDNKPCRAG CFVCRQSKQQ LEEEEKKALY GLENAEEMEE WQVTCGKFLA INATNMSCAC PRSPGGLSPF AHLGDGSSDL ILIRKCSRFN FLRFLIRHTN QEDQFDFTFV EVYRVKKFHF TSKHVEDEDN DSKEQEKQKF GKICKDRPSC TCSASRSSWN CDGEVMHSPA IEVRVHCQLV RLFARGIEEE S -
Predicted molecular weight
61 kDa including tags -
Amino acids
1 to 531 -
Tags
DDDDK tag C-Terminus -
Additional sequence information
Genbank: NM_00113486
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Preparation and Storage
- Acylsphingosine kinase
- Ceramide kinase
- Cerk
Images
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Functional Studies - Recombinant mouse CERK protein (Tagged) (ab271468)
Specific activity of ab271468 was ≥10 pmole/min/μg.
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SDS-PAGE - Recombinant mouse CERK protein (Tagged) (ab271468)SDS-PAGE analysis of ab271468.