Mouse FABP3 ELISA Kit (ab242240)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.8 pg/ml
- Range: 7.81 pg/ml - 500 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse FABP3 ELISA Kit
See all H-FABP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% heart 8 5.1% Inter-assay Sample n Mean SD CV% heart 3 5.7% -
Sample type
Urine, Serum, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.8 pg/ml -
Range
7.81 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 108 105% - 114% Serum 100 94% - 105% Tissue Extracts 110 104% - 116% Cell culture media 107 105% - 111% Hep Plasma 93 82% - 101% EDTA Plasma 99 97% - 101% Cit plasma 101 100% - 102% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse FABP3 ELISA Kit (ab242240) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FABP3 protein in cit plasma, edta plasma, hep plasma, serum, tissue extracts, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse FABP3 with 1.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse FABP3 Capture Antibody 1 x 600µl 10X Mouse FABP3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse FABP3 Lyophilized recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- 422 protein
- Cardiac FABP
- Cardiac Fatty Acid Binding Protein
see all -
Database links
- Entrez Gene: 14077 Mouse
- SwissProt: P11404 Mouse
- Unigene: 388886 Mouse
Images
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Other - Mouse FABP3 ELISA Kit (ab242240)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse FABP3 standard curve in Sample Diluent NS.The FABP3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse FABP3 standard curve in 1X Cell Extraction Buffer PTR.The FABP3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native FABP3 in mouse serum, plasma and urine samples.The concentrations of FABP3 were measured in duplicates, interpolated from the FABP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:200, plasma (citrate) 1:100, plasma (EDTA) 1:100, plasma (heparin) 1:100, and urine 1:8. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FABP3 concentration was determined to be 42.1 ng/mL in serum, 69.1 ng/mL in plasma (citrate), 57.2 ng/mL in plasma (EDTA), 76.5 ng/mL in plasma (heparin), and 3.4 ng/mL in urine.
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Interpolated concentrations of native FABP3 in mouse heart extract and skeletal muscle extract based on 100 ng/mL and 250 ng/mL extract loads, respectively.The concentrations of FABP3 were measured in duplicate and interpolated from the FABP3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FABP3 concentration was determined to be 427.5 pg/mL in heart extract and 344.3 pg/mL in skeletal muscle extract.
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Sandwich ELISA - Mouse FABP3 ELISA Kit (ab242240)To learn more about the advantages of recombinant antibodies see here.
