Anti-NRF1 antibody (ab34682)
Key features and details
- Rabbit polyclonal to NRF1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NRF1 antibody
See all NRF1 primary antibodies -
Description
Rabbit polyclonal to NRF1 -
Host species
Rabbit -
Specificity
ab34682 recognises NRF1 (also known as nuclear respiratory factor 1, alpha palindromic binding protein and alpha-pal). -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Chicken, Dog, Chimpanzee, Zebrafish
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Immunogen
Recombinant full length protein, corresponding to amino acids 1-534 of Mouse NRF1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NRF1 antibody (ab34682) This image is courtesy of an anonymous AbreviewAll lanes : Anti-NRF1 antibody (ab34682) at 1/1000 dilution
Lane 1 : Mouse liver whole tissue lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 55,67 kDa why is the actual band size different from the predicted?
Exposure time: 50 seconds
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Western blot - Anti-NRF1 antibody (ab34682)All lanes : Anti-NRF1 antibody (ab34682) at 1/500 dilution
Lane 1 : HeLa Nuclear extract
Lane 2 : HeLa Whole Cell lysate
Secondary
All lanes : IRDyeTM700 labelled goat-a-rabbit IgG at 1/2500 dilution
Predicted band size: 57 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody (ab34682)Purified anti-NRF1 antibody (ab34682) was used at 5µg/ml to detect NRF1 in human testis. Nuclear staining in lymphocytes can be observed. -
Immunocytochemistry/ Immunofluorescence - Anti-NRF1 antibody (ab34682)ICC/IF image of ab34682 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34682, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
