Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5554(N)] to NRF1 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt, ChIP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free
    See all NRF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5554(N)] to NRF1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP
    Human
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF-7, HeLa and 293T cell lysates and human fetal heart, mouse heart, mouse brain, rat heart and rat brain tissue lysates. IHC-P: Human gastric adenocarcinoma, human cervical carcinoma and human skeletal muscle tissues. ICC/IF: HeLa and MCF-7 cells. Flow Cyt: 293T cells. IP: 293T cell lysate.

  • General notes

    Ab221792 is the carrier-free version of ab175932. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab221792 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cevical carcinoma tissue labelling NRF1 with purified ab175932 at a dilution of 1/100. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175932).

  • Immunocytochemistry/ Immunofluorescence - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)
    Immunocytochemistry/ Immunofluorescence - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)

    Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling NRF1 with purified ab175932 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175932).

  • Flow Cytometry - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)
    Flow Cytometry - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)

    Flow Cytometry analysis of 293T cells labelling NRF1 with purified ab175932 at a dilution of 1/150 (red). Cells were fixed with 80% methanol. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175932).

  • ChIP - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)
    ChIP - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)

    Chromatin was prepared from Hela cells according to the Abcam Dual X-ChIP protocol. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab175932 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
    Primers and probes are located in the first kb of the transcribed region.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175932).

  • Immunoprecipitation - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)
    Immunoprecipitation - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)

    ab175932 (purified) at a dilution of 1/50 immunoprecipitating NRF1 in 293T whole cell lysate.

    Lane 1 (input): 293T whole cell lysate (10µg)

    Lane 2 (+): ab175932 + 293T whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab175932 in 293T whole cell lysate.

    For western blotting, ab131366 VeriBlot for IP (HRP) was used for detection at 1/1000 dilution.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175932).

  • ChIP - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)
    ChIP - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)

    Chromatin was prepared from NIH/3T3 treated with MG-132(2uM 16h) cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab175932 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
    Primers and probes are located in the first kb of the transcribed region.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175932).

  • Immunoprecipitation - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)
    Immunoprecipitation - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)

    ab175932 (unpurified) at a dilution of 1/10 immunoprecipitating NRF1 in 293T cell lysate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175932).

  • Flow Cytometry - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)
    Flow Cytometry - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)

    Flow cytometric analysis of permeabilized 293T cells labeling NRF1 with unpurified ab175932 at a dilution of 1/10 (red) compared to a negative control (rabbit IgG, green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175932).

  • Immunocytochemistry/ Immunofluorescence - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)
    Immunocytochemistry/ Immunofluorescence - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling NRF1 with unpurified ab175932 at a dilution of 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175932).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labeling NRF1 with unpurified ab175932 at a dilution of 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175932).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric adenocarcinoma tissue labeling NRF1 with unpurified ab175932 at a dilution of 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab175932).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)
    Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792)

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