Human CDKN2A ELISA Kit (ab227903)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.3 ng/ml
- Range: 2.3 ng/ml - 150 ng/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CDKN2A ELISA Kit
See all CDKN2A kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% HEK293T Ext 5 3.6% Inter-assay Sample n Mean SD CV% HEK293T Ext 3 3.4% -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
1.3 ng/ml -
Range
2.3 ng/ml - 150 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 116 110% - 125% Tissue Extracts 95 92% - 96% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CDKN2A ELISA Kit (ab227903) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CDKN2A protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CDKN2A with 1.3 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml 10X Human CDKN2A Capture Antibody 1 x 600µl 10X Human CDKN2A Detector Antibody 1 x 600µl Human CDKN2A Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein. Heterodimer with CDK4 or CDK6. Predominant p16 complexes contained CDK6. Interacts (isoforms 1,2 and 4) with CDK4 (both 'T-172'-phosphorylated and non-phosphorylated forms); the interaction inhibits cyclin D-CDK4 kinase activity. Interacts with ISCO2. Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific. -
Cellular localization
Cytoplasmic and Nuclear -
Alternative names
- Alternative reading frame
- ARF
- CDK4 inhibitor p16 INK4
see all -
Database links
- Entrez Gene: 1029 Human
- Omim: 600160 Human
- SwissProt: P42771 Human
- Unigene: 512599 Human
Images
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Other - Human CDKN2A ELISA Kit (ab227903)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of HumanCDKN2A standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CDKN2A in Human HeLa extract based on a 500 µg/mL extract loadThe concentrations of CDKN2A were measured in duplicate and interpolated from the CDKN2A standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CDKN2A concentration was determined to be 73 ng/mL in HeLa cell extract.
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Interpolated concentrations of spiked CDKN2A in Human Fetal Skeletal Muscle tissue homogenate extract based on a 250 µg/mL extract loadThe concentrations of CDKN2A were measured in duplicate and interpolated from the CDKN2A standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Human CDKN2A ELISA Kit (ab227903)To learn more about the advantages of recombinant antibodies see here.
