Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant human Wnt9b protein (Active) (ab245815)

Key features and details

  • Expression system: CHO cells
  • Purity: >= 95% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: HPLC, SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant human Wnt9b protein (Active)
    See all Wnt9b proteins and peptides

  • Biological activity

    Determined by its ability to induce alkaline phosphatase production by CCL-226 cells.

  • Purity

    >= 95 % SDS-PAGE.
    At least 95% by HPLC analysis.

  • Endotoxin level

  • Expression system

    CHO cells

  • Accession

    O14905

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      SYFGLTGREVLTPFPGLGTAAAPAQGGAHLKQCDLLKLSRRQKQLCRREP GLAETLRDAAHLGLLECQFQFRHERWNCSLEGRMGLLKRGFKETAFLYAV SSAALTHTLARACSAGRMERCTCDDSPGLESRQAWQWGVCGDNLKYSTKF LSNFLGSKRGNKDLRARADAHNTHVGIKAVKSGLRTTCKCHGVSGSCAVR TCWKQLSPFRETGQVLKLRYDSAVKVSSATNEALGRLELWAPARQGSLTK GLAPRSGDLVYMEDSPSFCRPSKYSPGTAGRVCSREASCSSLCCGRGYDT QSRLVAFSCHCQVQWCCYVECQQCVQEELVYTCKH

    • Predicted molecular weight

      37 kDa

    • Amino acids

      23 to 357

    • Additional sequence information

      Full-length mature chain lacking the signal peptide.

    • Specifications

    Our Abpromise guarantee covers the use of ab245815 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      HPLC

      SDS-PAGE

      Functional Studies

    • Form

      Lyophilized

    • Additional notes

      Due to glycosylation, ab245815 migrates at an apparent molecular weight of approximately 49-54 kDa by SDS-PAGE analysis under non-reducing conditions.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      Constituents: 0.12% Tris, 0.56% Potassium chloride, 1.45% Sodium chloride, 0.375% CHAPS

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      Centrifuge vial prior to opening. Reconstitute in water to 0.1-0.5 mg/ml. Do not vortex. Store at 4°C for 1 week, or prepare for extended storage. Follow reconstitution with further dilution in a buffer containing a carrier protein (example 0.1% BSA). Store working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.

    General Info

    • Alternative names

      • MGC124412
      • Protein Wnt 9b precursor
      • Protein Wnt-14b
      • Protein Wnt-9b
      • Wingless type MMTV integration site 9B
      • Wingless type MMTV integration site family member 15
      • Wingless type MMTV integration site family member 9B
      • Wnt 14b
      • Wnt-15
      • Wnt14b
      • Wnt15
      • Wnt9b wingless type MMTV integration site family member 9B
      see all

    • Relevance

      Wnt9b is a member of the WNT family and is a ligand for members of the frizzled family of seven transmembrane receptors. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. Wnt9b is expressed in the inductive epithelia and is essential for the development of mesonephric and metanephric tubules and caudal extension of the Müllerian duct. Wnt9b plays a central role in the regulation of mesenchymal to epithelial transitions underlying organogenesis of the mammalian urogenital system. The gene is clustered with WNT3, another family member, in the chromosome 17q21 region.

    • Cellular localization

      Secreted: extracellular space: extracellular matrix.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab245815? Please let us know so that we can cite the reference in this datasheet.

ab245815 has not yet been referenced specifically in any publications.

Preparation and Storage

  • MGC124412
  • Protein Wnt 9b precursor
  • Protein Wnt-14b

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