Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DHX36 antibody [EPR20868-12] (ab223564)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20868-12] to DHX36
  • Suitable for: IP, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-DHX36 antibody [EPR20868-12]
    See all DHX36 primary antibodies

  • Description

    Rabbit monoclonal [EPR20868-12] to DHX36

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse testis, heart, lung and brain tissue lysate, RAW264.7 and NIH/3T3 whole cell lysate, Wild-type mouse embryo heart tissue lysate, DHX36 knock-down mouse embryo heart tissue lysate. IP: RAW 264.7 cell lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. 

    For details on our patents, please refer to www.abcam.com/RabMAbPatents

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20868-12

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-DHX36 antibody [EPR20868-12] (ab223564)
    Western blot - Anti-DHX36 antibody [EPR20868-12] (ab223564)
    All lanes : Anti-DHX36 antibody [EPR20868-12] (ab223564) at 1/1000 dilution

    Lane 1 : Mouse testis tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 4 : Mouse lung tissue lysate
    Lane 5 : Mouse brain tissue lysate
    Lane 6 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 115 kDa
    Observed band size: 114 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST   

    Exposure time:

    Lanes 1-5: 3 minutes;

    Lane 6: 15 seconds.

  • Immunoprecipitation - Anti-DHX36 antibody [EPR20868-12] (ab223564)
    Immunoprecipitation - Anti-DHX36 antibody [EPR20868-12] (ab223564)

    DHX36 was immunoprecipitated from 0.35 mg RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab223564 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab223564 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10ug

    Lane 2: ab223564 IP in RAW 264.7 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223564 in RAW 264.7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes

     

  • Western blot - Anti-DHX36 antibody [EPR20868-12] (ab223564)
    Western blot - Anti-DHX36 antibody [EPR20868-12] (ab223564)
    All lanes : Anti-DHX36 antibody [EPR20868-12] (ab223564) at 1/1000 dilution

    Lane 1 : Wild-type mouse embryo heart tissue lysate
    Lane 2 : DHX36 knock-down mouse embryo heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 115 kDa
    Observed band size: 114 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lysates were kindly provided by Dr Zhongzhou Yang.

    ab223564 was shown to specifically react with DHX36 in wild-type mouse embryo heart tissue as signal was weaken in DHX36 knock-down tissue. Wild-type and DHX36 knock-down samples were subjected to SDS-PAGE. ab223564 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200, 000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/20, 000 dilution and 1/100, 000 dilution respectively for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    Exposure time: 15 seconds

  • Anti-DHX36 antibody [EPR20868-12] (ab223564)
    Anti-DHX36 antibody [EPR20868-12] (ab223564)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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