Anti-DHX36 antibody [EPR20868-12] (ab223564)
![Anti-DHX36 antibody [EPR20868-12] (ab223564)](https://www.abcam.com/ps/products/223/ab223564/Images/ab223564-387015-anti-dhx36-antibody-epr2086812-western-blot-mouse-testis-mouse-heart-raw2647-mouse-lungmouse-brain-nih3t3-lysates.jpg "Anti-DHX36 antibody [EPR20868-12] (ab223564)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20868-12] to DHX36
- Suitable for: IP, WB
- Reacts with: Mouse
Overview
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Product name
Anti-DHX36 antibody [EPR20868-12]
See all DHX36 primary antibodies -
Description
Rabbit monoclonal [EPR20868-12] to DHX36 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseWB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse testis, heart, lung and brain tissue lysate, RAW264.7 and NIH/3T3 whole cell lysate, Wild-type mouse embryo heart tissue lysate, DHX36 knock-down mouse embryo heart tissue lysate. IP: RAW 264.7 cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies.
For details on our patents, please refer to www.abcam.com/RabMAbPatents
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20868-12 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DHX36 antibody [EPR20868-12] (ab223564)All lanes : Anti-DHX36 antibody [EPR20868-12] (ab223564) at 1/1000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 4 : Mouse lung tissue lysate
Lane 5 : Mouse brain tissue lysate
Lane 6 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 115 kDa
Observed band size: 114 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time:
Lanes 1-5: 3 minutes;
Lane 6: 15 seconds.
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![Immunoprecipitation - Anti-DHX36 antibody [EPR20868-12] (ab223564)](https://www.abcam.com/ps/products/223/ab223564/Images/ab223564-1-anti-dhx36-antibody-epr2086812-immunoprecipitation-raw-2647.jpg)
Immunoprecipitation - Anti-DHX36 antibody [EPR20868-12] (ab223564)DHX36 was immunoprecipitated from 0.35 mg RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab223564 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab223564 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10ug
Lane 2: ab223564 IP in RAW 264.7 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223564 in RAW 264.7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
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![Western blot - Anti-DHX36 antibody [EPR20868-12] (ab223564)](https://www.abcam.com/ps/products/223/ab223564/Images/ab223564-387016-anti-dhx36-antibody-epr2086812-western-blot-wildtype-mouse-embryo-heart-dhx36-knockdown-mouse-embryo-heart-lysates.jpg)
Western blot - Anti-DHX36 antibody [EPR20868-12] (ab223564)All lanes : Anti-DHX36 antibody [EPR20868-12] (ab223564) at 1/1000 dilution
Lane 1 : Wild-type mouse embryo heart tissue lysate
Lane 2 : DHX36 knock-down mouse embryo heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 115 kDa
Observed band size: 114 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Lysates were kindly provided by Dr Zhongzhou Yang.
ab223564 was shown to specifically react with DHX36 in wild-type mouse embryo heart tissue as signal was weaken in DHX36 knock-down tissue. Wild-type and DHX36 knock-down samples were subjected to SDS-PAGE. ab223564 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200, 000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/20, 000 dilution and 1/100, 000 dilution respectively for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
Exposure time: 15 seconds
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![Anti-DHX36 antibody [EPR20868-12] (ab223564)](https://www.abcam.com/ps/products/223/ab223564/Images/ab223564-4-benefits-of-recombinant-antibodies.png)
Anti-DHX36 antibody [EPR20868-12] (ab223564)
