Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5528] to Chk2
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-Chk2 antibody [EPR5528]
See all Chk2 primary antibodies
Description

Rabbit monoclonal [EPR5528] to Chk2
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Chk2 aa 1-100 (N terminal). The exact sequence is proprietary.
Positive control
- SH-SY5Y, HeLa, HT29 and 293T cell lysates.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 1.02 x 10 ^-10 M
Learn more about K_D
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR5528
Isotype

IgG
Research areas

Western blot - Anti-Chk2 antibody [EPR5528] (ab133505)

Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 6 and 10: Chk2 knockout HAP1 cell lysate (20 µg)
Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
Lanes 4, 8 and 12: HEK293 cell lysate (20 µg)
Lanes 1, 2, 3 and 4: Green signal from target - ab133505 observed at 62 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control - ab8245 observed at 37 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signal
ab133505 was shown to specifically react with Chk2 when Chk2 knockout samples were used. Wild-type and Chk2 knockout samples were subjected to SDS-PAGE. ab133505 and ab8245 (loading control to GAPDH) were diluted 1/10000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-Chk2 antibody [EPR5528] (ab133505)

All lanes : Anti-Chk2 antibody [EPR5528] (ab133505) at 1/10000 dilution

Lane 1 : SH-SY5Y lysate
Lane 2 : HeLa lysate
Lane 3 : HT29 lysate
Lane 4 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 61 kDa
Observed band size: 61 kDa
Western blot - Anti-Chk2 antibody [EPR5528] (ab133505)

Lanes 1: Wild-type HAP1 cell lysate (20 µg)
Lanes 2: Chk2 knockout HAP1 cell lysate (20 µg)
Lanes 3: HeLa cell lysate (20 µg)
Lanes 4: HEK293 cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab133505 observed at 64 kDa. Red - loading control, ab8245, observed at 37 kDa.

This western blot image is a comparison between ab133505 and a competitor's rabbit polyclonal antibody.
OI-RD Scanning - Anti-Chk2 antibody [EPR5528] (ab133505)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-Chk2 antibody [EPR5528] (ab133505)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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