Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Chk2 (phospho T68) antibody [Y171] - BSA and Azide free (ab239808)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y171] to Chk2 (phospho T68) - BSA and Azide free
  • Suitable for: IP, Dot blot, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Chk2 (phospho T68) antibody [Y171] - BSA and Azide free
    See all Chk2 primary antibodies

  • Description

    Rabbit monoclonal [Y171] to Chk2 (phospho T68) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Cell lysates from HEK-293 cells treated with UV and/or phosphatase.

  • General notes

    ab239808 is the carrier-free version of ab32148 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab239808 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-Chk2 (phospho T68) antibody [Y171] - BSA and Azide free (ab239808)
    Immunoprecipitation - Anti-Chk2 (phospho T68) antibody [Y171] - BSA and Azide free (ab239808)

    ab32148 (purified) at 1:30 dilution (2µg) immunoprecipitating Chk2 in HEK-293 treated with UV whole cell lysate.
    Lane 1 (input): HEK-293 (Human embryonic kidney epithelial cell) treated with UV whole cell lysate 10µg
    Lane 2 (+): ab32148 & HEK-293 treated with UV whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32148 in HEK-293 treated with UV whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32148).

  • Immunoprecipitation - Anti-Chk2 (phospho T68) antibody [Y171] - BSA and Azide free (ab239808)
    Immunoprecipitation - Anti-Chk2 (phospho T68) antibody [Y171] - BSA and Azide free (ab239808)

    ab32148 at 1/20 dilution immunoprecipitating Chk2 (phospho T68) in HEK-293 (human embryonic kidney) whole cell lysate observed at 70kDa (lanes 1 and 2).

    Lane 1 (input): HEK-293 cells treated with UV at 60J/m2 for 2 hours. Whole cell lysate 10μg

    Lane 2 (+): ab32148 + HEK-293 treated with UV at 60J/m2 for 2 hours. Whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32148 in HEK- treated with UV at 60J/m2 for 2 hours. Whole cell lysate

    For western blotting, ab32148 at 1/500 dilution was used followed by VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Blocking and Diluting buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32148).

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32148).

  • Dot Blot - Anti-Chk2 (phospho T68) antibody [Y171] - BSA and Azide free (ab239808)
    Dot Blot - Anti-Chk2 (phospho T68) antibody [Y171] - BSA and Azide free (ab239808)

    Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using anti-Phospho-Chkr2 (pT68) RabMAb (ab32148) dilution 1:500

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32148).

    This image was generated using the unpurified version of the product. 

  • Anti-Chk2 (phospho T68) antibody [Y171] - BSA and Azide free (ab239808)
    Anti-Chk2 (phospho T68) antibody [Y171] - BSA and Azide free (ab239808)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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