Anti-Chk2 (phospho T68) antibody (ab3501)
Key features and details
- Rabbit polyclonal to Chk2 (phospho T68)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Chk2 (phospho T68) antibody
See all Chk2 primary antibodies -
Description
Rabbit polyclonal to Chk2 (phospho T68) -
Host species
Rabbit -
Specificity
ab3501 was tested using MCF7 cells treated with different amounts of doxorubicin (a DNA damaging agent). As the treatment increased the WB signal at 60kDa representing Chk2 increased, indicating that ab3501 was detecting the phosphorylated form of the protein. It has also been shown by peptide ELISA to be specific for the phosphorylated form of the immunizing peptide versus the non-phosphorylated form. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide: (C)ETVSTpQELYS, corresponding to amino acids 64-73 of Chk2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Chk2 (phospho T68) antibody (ab3501)
The Western blot shows lysates from MCF-7 cells treated for 24 hours with increasing concentrations of the DNA damaging agent Doxorubicin (0-10µM). The blot clearly shows that Chk2 phosphorylation is increased with the higher concentrations of Doxorubicin.
