Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Chk2 antibody [EPR19236] (ab199031)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19236] to Chk2
Suitable for: IHC-P, WB, IP
Reacts with: Mouse

Product name

Anti-Chk2 antibody [EPR19236]
See all Chk2 primary antibodies
Description

Rabbit monoclonal [EPR19236] to Chk2
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse spleen and colon lysates; NIH/3T3, C2C12 and bEND.3 whole cell lysates. IHC: Mouse spleen and testis tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19236
Isotype

IgG
Research areas

Western blot - Anti-Chk2 antibody [EPR19236] (ab199031)

All lanes : Anti-Chk2 antibody [EPR19236] (ab199031) at 1/1000 dilution

Lane 1 : C2C12 (mouse myoblast cell line) whole cell lysate
Lane 2 : bEND.3 (mouse brain endothelioma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 61 kDa
Observed band size: 61 kDa

Exposure time: 15 seconds

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Immunoprecipitation - Anti-Chk2 antibody [EPR19236] (ab199031)

Chk2 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate with ab199031 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab199031 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Exposure time 3 minutes.

Lane 1: NIH/3T3 whole cell lysate 10 µg (Input).

Lane 2: ab199031 IP in NIH/3T3 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199031 in NIH/3T3 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

All lanes : Anti-Chk2 antibody [EPR19236] (ab199031) at 1/1000 dilution

All lanes :

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Observed band size: 61 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk2 antibody [EPR19236] (ab199031)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Chk2 with ab199031 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101, ready to use). Nuclear staining on spermatogonia of mouse testis is observed (PMID: 12192050, 11593395). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB ab209101, ready to use.

Nuclear staining on spermatogonia of mouse testis (PMID: 12192050, PMID: 11593395).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk2 antibody [EPR19236] (ab199031)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Chk2 with ab199031 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101, ready to use). Nuclear staining on mouse spleen is observed (PMID: 12192050, 11593395). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB ab209101, ready to use.

Nuclear staining on mouse spleen (PMID: 12192050, PMID: 11593395).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-Chk2 antibody [EPR19236] (ab199031)

All lanes : Anti-Chk2 antibody [EPR19236] (ab199031) at 1/1000 dilution

Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse colon tissue lysate
Lane 3 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 61 kDa
Observed band size: 61 kDa

Exposure time: 3 minutes

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-Chk2 antibody [EPR19236] (ab199031)

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