Purified ab77232 staining Myoglobin in Human cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

Purified ab77232 staining Myoglobin in Rat cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

Purified ab77232 staining Myoglobin in the C2C12 (mouse myoblasts myoblast) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol. Samples were incubated with primary antibody and an Alexa Fluor^® 488-conjugated Goat anti-rabbit IgG (ab150077) was used as the secondary antibody. Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) and DAPI.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

Unpurified ab77232, at a 1/250 dilution, staining Myoglobin in paraffin embedded human heart muscle by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

Unpurified ab77232 showing positive staining in Skeletal muscle tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

Unpurified ab77232 showing negative staining in Normal brain tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

Unpurified ab77232 showing negative staining in Normal kidney tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

Unpurified ab77232 showing negative staining in Normal liver tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

Produced using unpurified ab77232.

Equilibrium disassociation constant (K_D)
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This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

Purified ab77232 staining Myoglobin in mouse cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A ready to use HRP-conjugated Goat Anti-Rabbit IgG H&L was used as the secondary antibody. Counterstained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).