Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP3081Y] to Myoglobin - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free
    See all Myoglobin primary antibodies

  • Description

    Rabbit monoclonal [EP3081Y] to Myoglobin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse cardiac muscle tissue.

  • General notes

    Ab232388 is the carrier-free version of ab77232. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232388 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)

    Purified ab77232 staining Myoglobin in Human cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)

    Purified ab77232 staining Myoglobin in Rat cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

  • Immunocytochemistry/ Immunofluorescence - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)
    Immunocytochemistry/ Immunofluorescence - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)

    Purified ab77232 staining Myoglobin in the C2C12 (mouse myoblasts myoblast) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol. Samples were incubated with primary antibody and an Alexa Fluor® 488-conjugated Goat anti-rabbit IgG (ab150077) was used as the secondary antibody. Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) and DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)

    Unpurified ab77232, at a 1/250 dilution, staining Myoglobin in paraffin embedded human heart muscle by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)

    Unpurified ab77232 showing positive staining in Skeletal muscle tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)

    Unpurified ab77232 showing negative staining in Normal brain tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)

    Unpurified ab77232 showing negative staining in Normal kidney tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)

    Unpurified ab77232 showing negative staining in Normal liver tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

  • OI-RD Scanning - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)
    OI-RD Scanning - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)

    Produced using unpurified ab77232.

    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)

    Purified ab77232 staining Myoglobin in mouse cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A ready to use HRP-conjugated Goat Anti-Rabbit IgG H&L was used as the secondary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77232).

  • Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)
    Anti-Myoglobin antibody [EP3081Y] - BSA and Azide free (ab232388)

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