Anti-Band 3/AE 1 antibody [EPR1425(2)] - BSA and Azide free (ab249866)
![Anti-Band 3/AE 1 antibody [EPR1425(2)] - BSA and Azide free (ab249866)](https://www.abcam.com/ps/products/249/ab249866/Images/ab249866-3-anti-band-3-ae-1-antibody-epr1425-2-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg "Anti-Band 3/AE 1 antibody [EPR1425(2)] - BSA and Azide free (ab249866)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1425(2)] to Band 3/AE 1 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-Band 3/AE 1 antibody [EPR1425(2)] - BSA and Azide free
See all Band 3/AE 1 primary antibodies -
Description
Rabbit monoclonal [EPR1425(2)] to Band 3/AE 1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: ICC or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249866 is the carrier-free version of ab175214 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab249866 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR1425(2) -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1425(2)] - BSA and Azide free (ab249866)This data was developed using ab175214, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Band 3/AE 1 at 1/250 dilution. Note erythrocyte staining. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1425(2)] - BSA and Azide free (ab249866)](https://www.abcam.com/ps/products/249/ab249866/Images/ab249866-2-anti-band-3-ae-1-antibody-epr1425-2-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1425(2)] - BSA and Azide free (ab249866)This data was developed using ab175214, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Band 3/AE 1 wtih ab175214 at 1/250 dilution. Note erythrocyte staining. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Western blot - Anti-Band 3/AE 1 antibody [EPR1425(2)] - BSA and Azide free (ab249866)](https://www.abcam.com/ps/products/249/ab249866/Images/ab249866-398832-anti-band-3-ae-1-antibody-epr1425-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Band 3/AE 1 antibody [EPR1425(2)] - BSA and Azide free (ab249866) -
![Anti-Band 3/AE 1 antibody [EPR1425(2)] - BSA and Azide free (ab249866)](https://www.abcam.com/ps/products/249/ab249866/Images/ab249866-4-benefits-of-recombinant-antibodies.png)
Anti-Band 3/AE 1 antibody [EPR1425(2)] - BSA and Azide free (ab249866)
