Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CEBP Delta/CEBPD antibody [EPR23518-259] - BSA and Azide free (ab270410)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23518-259] to CEBP Delta/CEBPD - BSA and Azide free
  • Suitable for: IHC-P, WB, IHC-Fr, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CEBP Delta/CEBPD antibody [EPR23518-259] - BSA and Azide free
    See all CEBP Delta/CEBPD primary antibodies

  • Description

    Rabbit monoclonal [EPR23518-259] to CEBP Delta/CEBPD - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IHC-Fr, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: ,Caco-2, HaCaT and differentiated NIH/3T3 L1 cell lysates. IHC-P: Mouse hippocampus tissue; Rat cerebrum tissue. IHC-Fr: Mouse hippocampus tissue; Rat hippocampus tissue. IP: Differentiated NIH/3T3-L1 cells.

  • General notes

    ab270410 is the carrier-free version of ab245214.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] - BSA and Azide free (ab270410)
    Immunoprecipitation - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] - BSA and Azide free (ab270410)

    CEBP Delta/CEBPD was immunoprecipitated from 0.35 mg differentiated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate with ab245214 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using 245214 1/1000 dilution (0.518 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Differentiated 3T3-L1 (mouse embryonic fibroblast), whole cell lysate 10 μg

    Lane 2: ab245214 IP in Differentiated 3T3-L1 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245214 in differentiated 3T3-L1 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 180 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245214).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] - BSA and Azide free (ab270410)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] - BSA and Azide free (ab270410)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling CEBP Delta/CEBPD with ab245214 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and weak cytoplasmic staining on rat cerebrum is observed. The section was incubated with ab245214 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245214).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] - BSA and Azide free (ab270410)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] - BSA and Azide free (ab270410)

    Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling CEBP Delta/CEBPD with ab245214 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse hippocampus is observed. The section was incubated with ab245214 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245214).

  • Immunohistochemistry (Frozen sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] - BSA and Azide free (ab270410)
    Immunohistochemistry (Frozen sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] - BSA and Azide free (ab270410)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus tissue labeling CEBP Delta/CEBPD with ab245214 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution (Green). The nuclear counterstain was DAPI (Blue). Nuclear staining on rat hippocampus is observed.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245214).

  • Immunohistochemistry (Frozen sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] - BSA and Azide free (ab270410)
    Immunohistochemistry (Frozen sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] - BSA and Azide free (ab270410)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus tissue labeling CEBP Delta/CEBPD with ab245214 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution (Green). The nuclear counterstain was DAPI (Blue). Nuclear staining on mouse hippocampus is observed.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245214).

  • Anti-CEBP Delta/CEBPD antibody [EPR23518-259] - BSA and Azide free (ab270410)
    Anti-CEBP Delta/CEBPD antibody [EPR23518-259] - BSA and Azide free (ab270410)

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