Anti-Cytosolic Phospholipase A2 antibody (ab139388)
Key features and details
- Rabbit polyclonal to Cytosolic Phospholipase A2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cytosolic Phospholipase A2 antibody
See all Cytosolic Phospholipase A2 primary antibodies -
Description
Rabbit polyclonal to Cytosolic Phospholipase A2 -
Host species
Rabbit -
Specificity
ab139388 detects endogenous levels of total Cytosolic Phospholipase A2 protein. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human Cytosolic Phospholipase A2.
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Positive control
- Human uterus and tonsil tissues; HeLa cells treated with TNF alpha; HuvEc cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Cytosolic Phospholipase A2 antibody (ab139388) at 1/500 dilution
Lane 1 : HeLa cell lysate treated with TNF alpha at 20 ng/ml for 30 minutes
Lane 2 : HeLa cell lysate treated with TNF alpha at 20 ng/ml for 30 minutes, and also treated with the synthesized peptide
Predicted band size: 85 kDa
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Immunohistochemical analysis of formalin fixed, paraffin embedded Human tonsil tissue labeled with ab139388 at 10 µg/ml.
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Immunohistochemical analysis of formalin fixed, paraffin embedded Human uterus tissue labeled with ab139388 at 10 µg/ml.
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Immunofluorescence analysis of HuvEc cells labeling Cytosolic Phospholipase A2 with ab139388 at a 1/100 dilution. The picture on the right is treated with the synthesized peptide.