Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] (ab191605)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18552] to Cytosolic Phospholipase A2 (phospho S505)
  • Suitable for: Dot blot, IP, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552]
    See all Cytosolic Phospholipase A2 primary antibodies

  • Description

    Rabbit monoclonal [EPR18552] to Cytosolic Phospholipase A2 (phospho S505)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • NIH/3T3 treated with 20 ng/ml TNF-alpha for 10 minutes whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] (ab191605)
    Western blot - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] (ab191605)
    All lanes : Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] (ab191605) at 1/2000 dilution

    Lane 1 : Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) treated with 20 ng/ml TNF-alpha for 10 minutes whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 85 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] (ab191605)
    Immunoprecipitation - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] (ab191605)

    ab191605 at 1/50 dilution immunoprecipitating Cytosolic  Phospholipase A2 (phospho S505) in NIH/3T3 (mouse embryo) whole cell lysate.

    Lane 1 (input): NIH/3T3 cells treated with 20ng/ml TNF-a for 60min whole cell lysate 10μg

    Lane 2 (+): ab191605 + NIH/3T3 cells treated with 20ng/ml TNF-a for 60min whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab191605 in NIH/3T3 cells treated with 20ng/ml TNF-a for 60min whole cell lysate

    For western blotting, ab191605 1/1000 dilution and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Blocking and Diluting buffer and concentration: 5% NFDM/TBST.

  • Dot Blot - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] (ab191605)
    Dot Blot - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] (ab191605)

    Dot blot analysis of Cytosolic Phospholipase A2 (phospho S505) phospho peptide (Lane 1) and Phospholipase A2 Non-phospho peptide (Lane 2) labeled using ab191605 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100000 dilution.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] (ab191605)
    Anti-Cytosolic Phospholipase A2 (phospho S505) antibody [EPR18552] (ab191605)

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