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Immunology Innate Immunity Macrophage / Inflamm.

Anti-NLRC4 antibody [EPR19733] (ab201792)

Price and availability

288 134 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-NLRC4 antibody [EPR19733] (ab201792)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19733] to NLRC4
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-NLRC4 antibody [EPR19733]
    See all NLRC4 primary antibodies
  • Description

    Rabbit monoclonal [EPR19733] to NLRC4
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 293T whole cell lysate transfected with mouse NLRC4; RAW 264.7 overexpressed with ASC whole cell lysate.
  • General notes

     This product was previously labelled as CARD12

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19733
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • CARD Family
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Images

  • Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)
    Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)
    Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/200 dilution + MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 117 kDa
    Observed band size: 117 kDa


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)
    Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)
    All lanes : Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate (Human epithelial cell line from embryonic kidney)
    Lane 2 : 293T whole cell lysate transfected with mouse NLRC4

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 117 kDa
    Observed band size: 117 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 0.5 second.

    The cell lysates were kindly provided by our collaborator Dr. Jiahuai Han, Xiamen University

  • Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)
    Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)
    Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/1000 dilution + Mouse spleen tissue lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 117 kDa
    Observed band size: 117 kDa


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)
    Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792)
    All lanes : Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/1000 dilution

    Lane 1 : RAW 264.7 whole cell lysate (Mouse macrophage cell line transformed with Abelson murine leukemia virus) overexpressed with ASC
    Lane 2 : RAW 264.7 whole cell lysate NLRC4 KO overexpressed with ASC

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 117 kDa
    Observed band size: 117 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    NLRC4 regulation involves mechanisms that require ASC (apoptosis-associated speck-like protein containing a CARD), a common adaptor protein for several inflammasomes - PMID: 25775556.
    The cell lysates were kindly provided by our Collaborator Dr. Jiahuai Han, Xiamen University

  • Anti-NLRC4 antibody [EPR19733] (ab201792)
    Anti-NLRC4 antibody [EPR19733] (ab201792)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-NLRC4 antibody [EPR19733] (ab201792)

  •  
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    Applications: ICC/IF, WB

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    Anti-NLRC4 antibody [EPR19733] - BSA and Azide free (ab223143)

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    Applications: IHC-P, WB

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