Anti-NLRC4 antibody [EPR19733] (ab201792)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19733] to NLRC4
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Overview
-
Product name
Anti-NLRC4 antibody [EPR19733]
See all NLRC4 primary antibodies -
Description
Rabbit monoclonal [EPR19733] to NLRC4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: 293T whole cell lysate transfected with mouse NLRC4; RAW 264.7 overexpressed with ASC whole cell lysate.
-
General notes
This product was previously labelled as CARD12
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19733 -
Isotype
IgG -
Research areas
Images
-
Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/200 dilution + MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDa
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST
-
All lanes : Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/1000 dilution
Lane 1 : 293T whole cell lysate (Human epithelial cell line from embryonic kidney)
Lane 2 : 293T whole cell lysate transfected with mouse NLRC4
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 0.5 second.
The cell lysates were kindly provided by our collaborator Dr. Jiahuai Han, Xiamen University
-
Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/1000 dilution + Mouse spleen tissue lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDa
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST
-
All lanes : Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/1000 dilution
Lane 1 : RAW 264.7 whole cell lysate (Mouse macrophage cell line transformed with Abelson murine leukemia virus) overexpressed with ASC
Lane 2 : RAW 264.7 whole cell lysate NLRC4 KO overexpressed with ASC
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
NLRC4 regulation involves mechanisms that require ASC (apoptosis-associated speck-like protein containing a CARD), a common adaptor protein for several inflammasomes - PMID: 25775556.
The cell lysates were kindly provided by our Collaborator Dr. Jiahuai Han, Xiamen University -